Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2494960..2495522 Replicon chromosome
Accession NZ_CP051725
Organism Escherichia coli strain SCU-112

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag HHJ29_RS11665 Protein ID WP_000605675.1
Coordinates 2494960..2495238 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag HHJ29_RS11670 Protein ID WP_000781370.1
Coordinates 2495238..2495522 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ29_RS11640 2490546..2490977 - 432 WP_000152307.1 peroxiredoxin OsmC -
HHJ29_RS11645 2491322..2491537 + 216 WP_000495766.1 biofilm-dependent modulation protein -
HHJ29_RS11650 2491639..2491776 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
HHJ29_RS11655 2491933..2493630 + 1698 WP_000433460.1 malate dehydrogenase -
HHJ29_RS11660 2493764..2494774 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
HHJ29_RS11665 2494960..2495238 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HHJ29_RS11670 2495238..2495522 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
HHJ29_RS11675 2495751..2496404 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
HHJ29_RS11680 2496397..2497281 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
HHJ29_RS11685 2497294..2500341 - 3048 WP_011076399.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T155058 WP_000605675.1 NZ_CP051725:2494960-2495238 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T155058 NZ_CP068614:c5419978-5419595 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT155058 WP_000781370.1 NZ_CP051725:2495238-2495522 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT155058 NZ_CP068614:c5420220-5419978 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References