Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1172257..1172984 Replicon chromosome
Accession NZ_CP051725
Organism Escherichia coli strain SCU-112

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag HHJ29_RS05580 Protein ID WP_000547555.1
Coordinates 1172257..1172568 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H2V9Z2
Locus tag HHJ29_RS05585 Protein ID WP_000126293.1
Coordinates 1172565..1172984 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ29_RS05550 1167398..1169107 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
HHJ29_RS05555 1169117..1169659 + 543 WP_000493797.1 formate hydrogenlyase subunit HycF -
HHJ29_RS05560 1169659..1170426 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
HHJ29_RS05565 1170423..1170833 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
HHJ29_RS05570 1170826..1171296 + 471 WP_000132956.1 hydrogenase maturation peptidase HycI -
HHJ29_RS05575 1171339..1172094 + 756 WP_001305268.1 hypothetical protein -
HHJ29_RS05580 1172257..1172568 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
HHJ29_RS05585 1172565..1172984 + 420 WP_000126293.1 helix-turn-helix domain-containing protein Antitoxin
HHJ29_RS05590 1173076..1173504 - 429 WP_000536064.1 hypothetical protein -
HHJ29_RS05595 1173889..1174416 + 528 WP_001078777.1 electron transport protein HydN -
HHJ29_RS05600 1174569..1176821 + 2253 WP_001107659.1 carbamoyltransferase HypF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T155046 WP_000547555.1 NZ_CP051725:1172257-1172568 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T155046 NZ_CP068610:c5276979-5276596 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15426.46 Da        Isoelectric Point: 4.4596

>AT155046 WP_000126293.1 NZ_CP051725:1172565-1172984 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTIPHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT155046 NZ_CP068610:c5277221-5276979 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H2V9Z2

References