Detailed information of TA system TA155036

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 4229555..4230234
• Replicon: chromosome
• Accession: NZ_CP051719
• Organism: Escherichia coli strain SCU-116

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0VBP6
• Locus tag: HHJ31_RS20240
• Protein ID: WP_000057524.1
• Coordinates: 4229555..4229857 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: S1QAY3
• Locus tag: HHJ31_RS20245
• Protein ID: WP_000806442.1
• Coordinates: 4229893..4230234 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ31_RS20215	4224931..4226583	+	1653	WP_021531972.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-
HHJ31_RS20220	4226621..4227124	-	504	WP_000667000.1	hypothetical protein	-
HHJ31_RS20225	4227121..4227921	-	801	WP_000439796.1	hypothetical protein	-
HHJ31_RS20230	4227945..4228424	-	480	WP_000186633.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
HHJ31_RS20235	4228628..4229422	-	795	WP_000365177.1	TraB/GumN family protein	-
HHJ31_RS20240	4229555..4229857	+	303	WP_000057524.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HHJ31_RS20245	4229893..4230234	+	342	WP_000806442.1	HigA family addiction module antidote protein	Antitoxin
HHJ31_RS20250	4230292..4232796	-	2505	WP_000083945.1	copper-exporting P-type ATPase CopA	-
HHJ31_RS20255	4233058..4233990	+	933	WP_000883052.1	glutaminase A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4226621..4238835	12214

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-100)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11839.47 Da
• Isoelectric Point: 10.2237

>T155036 WP_000057524.1 NZ_CP051719:4229555-4229857 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Nucleotide

• Length: 303 bp

>T155036 NZ_CP068610:323405-323773 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13171.10 Da
• Isoelectric Point: 5.7790

>AT155036 WP_000806442.1 NZ_CP051719:4229893-4230234 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Nucleotide

• Length: 342 bp

>AT155036 NZ_CP068610:323187-323408 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: V0VBP6

Antitoxin

• AlphaFold DB: S1QAY3