Detailed information of TA system TA155024

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3017867..3018469
• Replicon: chromosome
• Accession: NZ_CP051719
• Organism: Escherichia coli strain SCU-116

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: HHJ31_RS14485
• Protein ID: WP_000897302.1
• Coordinates: 3017867..3018178 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HHJ31_RS14490
• Protein ID: WP_000356397.1
• Coordinates: 3018179..3018469 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ31_RS14460	3013781..3014380	+	600	WP_001314324.1	glucose-1-phosphatase	-
HHJ31_RS14465	3014374..3015246	+	873	WP_000920762.1	virulence factor BrkB family protein	-
HHJ31_RS14470	3015243..3015680	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HHJ31_RS14475	3015725..3016666	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HHJ31_RS14480	3016730..3017638	-	909	WP_001331553.1	alpha/beta hydrolase	-
HHJ31_RS14485	3017867..3018178	+	312	WP_000897302.1	hypothetical protein	Toxin
HHJ31_RS14490	3018179..3018469	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HHJ31_RS14495	3018828..3019106	+	279	WP_001296612.1	hypothetical protein	-
HHJ31_RS14500	3019502..3019720	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
HHJ31_RS14505	3019936..3020865	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
HHJ31_RS14510	3020862..3021497	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HHJ31_RS14515	3021494..3022396	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T155024 WP_000897302.1 NZ_CP051719:3017867-3018178 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T155024 NZ_CP068602:738018-738503 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT155024 WP_000356397.1 NZ_CP051719:3018179-3018469 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT155024 NZ_CP068602:737728-738021 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Toxin

• AlphaFold DB: S1P416