Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3578963..3579657 Replicon chromosome
Accession NZ_CP051716
Organism Escherichia coli strain SCU-118

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag HHJ32_RS17205 Protein ID WP_001263489.1
Coordinates 3578963..3579361 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag HHJ32_RS17210 Protein ID WP_000554758.1
Coordinates 3579364..3579657 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 3574551..3574631 - 81 NuclAT_11 - -
- 3574551..3574631 - 81 NuclAT_11 - -
- 3574551..3574631 - 81 NuclAT_11 - -
- 3574551..3574631 - 81 NuclAT_11 - -
HHJ32_RS17180 3575227..3575685 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
HHJ32_RS17185 3575946..3577403 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
HHJ32_RS17190 3577460..3577981 - 522 Protein_3358 peptide chain release factor H -
HHJ32_RS17195 3577977..3578183 - 207 Protein_3359 RtcB family protein -
HHJ32_RS17200 3578501..3578953 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
HHJ32_RS17205 3578963..3579361 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
HHJ32_RS17210 3579364..3579657 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
HHJ32_RS17215 3579709..3580764 - 1056 WP_001226164.1 DNA polymerase IV -
HHJ32_RS17220 3580835..3581620 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
HHJ32_RS17225 3581592..3583304 + 1713 Protein_3365 flagellar biosynthesis protein FlhA -
HHJ32_RS17230 3583528..3584025 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3556497..3584827 28330


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T154998 WP_001263489.1 NZ_CP051716:c3579361-3578963 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T154998 NZ_CP068591:714778-715128 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT154998 WP_000554758.1 NZ_CP051716:c3579657-3579364 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT154998 NZ_CP068591:714533-714784 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References