Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1783744..1784246 Replicon chromosome
Accession NZ_CP051716
Organism Escherichia coli strain SCU-118

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HHJ32_RS08375 Protein ID WP_000767829.1
Coordinates 1783992..1784246 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A6N8RC01
Locus tag HHJ32_RS08370 Protein ID WP_001259251.1
Coordinates 1783744..1783995 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ32_RS08345 1778921..1779988 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
HHJ32_RS08350 1779988..1781058 - 1071 WP_032082632.1 histidinol-phosphate transaminase -
HHJ32_RS08355 1781055..1782359 - 1305 WP_032082631.1 histidinol dehydrogenase -
HHJ32_RS08360 1782365..1783264 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
HHJ32_RS08365 1783410..1783460 - 51 WP_001364200.1 his operon leader peptide -
HHJ32_RS08370 1783744..1783995 + 252 WP_001259251.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HHJ32_RS08375 1783992..1784246 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HHJ32_RS08380 1784329..1785153 + 825 WP_000754737.1 SDR family oxidoreductase -
HHJ32_RS08385 1785199..1786128 + 930 WP_000803351.1 LysR family transcriptional regulator -
HHJ32_RS08390 1786343..1786405 + 63 WP_010723108.1 membrane protein YoeI -
HHJ32_RS08395 1786395..1787753 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
HHJ32_RS08400 1787932..1788990 + 1059 WP_000492340.1 transport protein YeeE -
HHJ32_RS08405 1789004..1789231 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T154985 WP_000767829.1 NZ_CP051716:1783992-1784246 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T154985 NZ_CP068572:c4348630-4348145 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9293.56 Da        Isoelectric Point: 4.7815

>AT154985 WP_001259251.1 NZ_CP051716:1783744-1783995 [Escherichia coli]
MRTISYSEARQNLSATMMKAVDDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT154985 NZ_CP068572:c4348920-4348627 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A6N8RC01

References