Detailed information of TA system TA154979

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4697418..4698020
• Replicon: chromosome
• Accession: NZ_CP051714
• Organism: Escherichia coli strain SCU-122

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: HHJ35_RS22415
• Protein ID: WP_000897302.1
• Coordinates: 4697709..4698020 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HHJ35_RS22410
• Protein ID: WP_000356397.1
• Coordinates: 4697418..4697708 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ35_RS22380	4693491..4694393	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HHJ35_RS22385	4694390..4695025	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HHJ35_RS22390	4695022..4695951	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
HHJ35_RS22395	4696167..4696385	-	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
HHJ35_RS22400	4696781..4697059	-	279	WP_001296612.1	hypothetical protein	-
HHJ35_RS22405	4697121..4697333	-	213	WP_000197774.1	hypothetical protein	-
HHJ35_RS22410	4697418..4697708	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HHJ35_RS22415	4697709..4698020	-	312	WP_000897302.1	hypothetical protein	Toxin
HHJ35_RS22420	4698249..4699157	+	909	WP_001331553.1	alpha/beta hydrolase	-
HHJ35_RS22425	4699221..4700162	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HHJ35_RS22430	4700207..4700644	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HHJ35_RS22435	4700641..4701513	-	873	WP_000920762.1	virulence factor BrkB family protein	-
HHJ35_RS22440	4701507..4702106	-	600	WP_001314324.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T154979 WP_000897302.1 NZ_CP051714:c4698020-4697709 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T154979 NZ_CP068572:326891-327259 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT154979 WP_000356397.1 NZ_CP051714:c4697708-4697418 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT154979 NZ_CP068572:326673-326894 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: S1P416