Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3714636..3715330 Replicon chromosome
Accession NZ_CP051714
Organism Escherichia coli strain SCU-122

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag HHJ35_RS17770 Protein ID WP_001263500.1
Coordinates 3714636..3715034 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag HHJ35_RS17775 Protein ID WP_000554758.1
Coordinates 3715037..3715330 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ35_RS17745 3710001..3710459 - 459 WP_001291988.1 xanthine phosphoribosyltransferase -
HHJ35_RS17750 3710720..3712177 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
HHJ35_RS17755 3712234..3712848 - 615 WP_000602123.1 peptide chain release factor H -
HHJ35_RS17760 3712845..3713984 - 1140 WP_000521577.1 RNA ligase RtcB family protein -
HHJ35_RS17765 3714174..3714626 - 453 WP_001059895.1 GNAT family N-acetyltransferase -
HHJ35_RS17770 3714636..3715034 - 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
HHJ35_RS17775 3715037..3715330 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
HHJ35_RS17780 3715382..3716437 - 1056 WP_001226168.1 DNA polymerase IV -
HHJ35_RS17785 3716508..3717293 - 786 WP_000207544.1 putative lateral flagellar export/assembly protein LafU -
HHJ35_RS17790 3717265..3718977 + 1713 Protein_3474 flagellar biosynthesis protein FlhA -
HHJ35_RS17795 3719056..3719214 + 159 WP_014639450.1 hypothetical protein -
HHJ35_RS17800 3719297..3719794 - 498 WP_001557224.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3714636..3734990 20354


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T154970 WP_001263500.1 NZ_CP051714:c3715034-3714636 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T154970 NZ_CP068559:c1516695-1516543 [Clostridioides difficile]
ATGGATAATTTTTTACAAGGTATATTAGCAAGTTTAGTTGCCAGTTTAATAGTTTACTTAACTAGTAAGTTATTTAAAAA
AGTAAAAAGCCACTCTGGCAGGAGTGACTTTAGTTTTGAACTAGAAATCAAGTTCAAAAAGAATAAACATTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT154970 WP_000554758.1 NZ_CP051714:c3715330-3715037 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT154970 NZ_CP068559:1516499-1516599 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTAGACGTAGAGTGAAGTTCAAATACTAATGTTTATTCTTTTTGAACTTGATTTCTAGTTC
AAAACTAAAGTCACTCCTGCC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References