Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3485716..3486395 Replicon chromosome
Accession NZ_CP051714
Organism Escherichia coli strain SCU-122

Toxin (Protein)


Gene name higB Uniprot ID V0VBP6
Locus tag HHJ35_RS16710 Protein ID WP_000057524.1
Coordinates 3486093..3486395 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag HHJ35_RS16705 Protein ID WP_000806442.1
Coordinates 3485716..3486057 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ35_RS16695 3481960..3482892 - 933 WP_000883052.1 glutaminase A -
HHJ35_RS16700 3483154..3485658 + 2505 WP_001557230.1 copper-exporting P-type ATPase CopA -
HHJ35_RS16705 3485716..3486057 - 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
HHJ35_RS16710 3486093..3486395 - 303 WP_000057524.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HHJ35_RS16715 3486528..3487322 + 795 WP_000365177.1 TraB/GumN family protein -
HHJ35_RS16720 3487526..3488005 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
HHJ35_RS16725 3488029..3488829 + 801 WP_000439796.1 hypothetical protein -
HHJ35_RS16730 3488826..3489329 + 504 WP_000667000.1 hypothetical protein -
HHJ35_RS16735 3489367..3491019 - 1653 WP_000771762.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3478405..3489329 10924


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11839.47 Da        Isoelectric Point: 10.2237

>T154968 WP_000057524.1 NZ_CP051714:c3486395-3486093 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T154968 NZ_CP068556:1049364-1049516 [Clostridioides difficile]
ATGGATAATTTTTTACAAGGTATATTAGCAAGTTTAGTTGCCAGTTTAATAGTTTACTTAACTAGTAAGTTATTTAAAAA
AGTAAAAAGCCACTCTGGCAGGAGTGACTTTAGTTTTGAACTAGAAATCAAGTTCAAAAAGAATAAACATTAG

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT154968 WP_000806442.1 NZ_CP051714:c3486057-3485716 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT154968 NZ_CP068556:c1049560-1049460 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTAGACGTAGAGTGAAGTTCAAATACTAATGTTTATTCTTTTTGAACTTGATTTCTAGTTC
AAAACTAAAGTCACTCCTGCC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VBP6

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References