Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 4205207..4205886 Replicon chromosome
Accession NZ_CP051711
Organism Escherichia coli strain SCU-123

Toxin (Protein)


Gene name higB Uniprot ID V0VBP6
Locus tag HHJ36_RS20040 Protein ID WP_000057524.1
Coordinates 4205207..4205509 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag HHJ36_RS20045 Protein ID WP_000806442.1
Coordinates 4205545..4205886 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ36_RS20015 4200583..4202235 + 1653 WP_000771762.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
HHJ36_RS20020 4202273..4202776 - 504 WP_000667000.1 hypothetical protein -
HHJ36_RS20025 4202773..4203573 - 801 WP_000439796.1 hypothetical protein -
HHJ36_RS20030 4203597..4204076 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
HHJ36_RS20035 4204280..4205074 - 795 WP_000365177.1 TraB/GumN family protein -
HHJ36_RS20040 4205207..4205509 + 303 WP_000057524.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HHJ36_RS20045 4205545..4205886 + 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
HHJ36_RS20050 4205944..4208448 - 2505 WP_000083945.1 copper-exporting P-type ATPase CopA -
HHJ36_RS20055 4208710..4209642 + 933 WP_000883052.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4202273..4214487 12214


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11839.47 Da        Isoelectric Point: 10.2237

>T154947 WP_000057524.1 NZ_CP051711:4205207-4205509 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T154947 NZ_CP068443:467746-468057 [Pantoea agglomerans]
ATGCATATCATCTCAAGAGGGCCGTTCAGGGAAGCGGCAATAATTTATCCAAATCATGCTTCCGCACTGGATGCGGCTTA
TCTGGTCCTCAGGGACGAAAACTTTGCCACGCCAGATGCGCTGAAAATTCGCTTTCAGAGCCTTGACCGCATGAAGTACC
GTGAAAAGTGGTGGGTAATCGATGTGGGCGGAAACAGCCTGAGGATCATGTTCTATGCTGACTTTGATCGAGGCAAAATT
TTTATCAAGCACATCGTGACGCATGCGGAATATGACAAGCTGGTGAAGAAGTACCGGGAGAGTAAAGAATGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT154947 WP_000806442.1 NZ_CP051711:4205545-4205886 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT154947 NZ_CP068443:468054-468467 [Pantoea agglomerans]
ATGATTACCGAAGCAATCCAGGCGACCAATGACCTGGTCAGAATTGTTCCCTTTCTGGGAGGCAGTACCGATAAAAGGGA
CTACGAGCAGGCGCTGGAACTGGTTGAATATCTGGTTGAGCACCAGCCGGACAGCCCGTTAGTAGAGATACTGTCTGATA
AGGTCGCCCGATACGAAAACAGCGCCCCTGAGTTTGCTGCGTTTAACGCGCGTACTGATGCTATGCCGCGCGGAGTGGCA
CTTTTGCGGGTCATCATGGATCAGCACGGCCTGACTCAATCCTCTTTTGCGGATGAAATAGGGCAGCGCTCTTATGTCAG
TCGTATCCTGCGTGGTGATCGCCCCCTGACGGATAAACATAAAGCCCGACTGGCAGCGCGTTTTAACCTGCCGTTTGAAG
CGTTCGCTGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VBP6

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References