Detailed information of TA system TA154945

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3976211..3976905
• Replicon: chromosome
• Accession: NZ_CP051711
• Organism: Escherichia coli strain SCU-123

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: HHJ36_RS18980
• Protein ID: WP_001263500.1
• Coordinates: 3976507..3976905 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: HHJ36_RS18975
• Protein ID: WP_000554758.1
• Coordinates: 3976211..3976504 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ36_RS18950	3971747..3972244	+	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-
HHJ36_RS18955	3972327..3972485	-	159	WP_014639450.1	hypothetical protein	-
HHJ36_RS18960	3972564..3974276	-	1713	Protein_3697	flagellar biosynthesis protein FlhA	-
HHJ36_RS18965	3974248..3975033	+	786	WP_000207544.1	putative lateral flagellar export/assembly protein LafU	-
HHJ36_RS18970	3975104..3976159	+	1056	WP_001226168.1	DNA polymerase IV	-
HHJ36_RS18975	3976211..3976504	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HHJ36_RS18980	3976507..3976905	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HHJ36_RS18985	3976915..3977367	+	453	WP_001059895.1	GNAT family N-acetyltransferase	-
HHJ36_RS18990	3977557..3978696	+	1140	WP_000521577.1	RNA ligase RtcB family protein	-
HHJ36_RS18995	3978693..3979307	+	615	WP_000602123.1	peptide chain release factor H	-
HHJ36_RS19000	3979364..3980821	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
HHJ36_RS19005	3981082..3981540	+	459	WP_001291988.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	3956686..3977367	20681

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T154945 WP_001263500.1 NZ_CP051711:3976507-3976905 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T154945 NZ_CP068443:241837-242307 [Pantoea agglomerans]
TTGATTCTCTACAGGCTGACCAAGACAAAGTATCTTTCGACAGCCTGGACGGGATTTGGCGCAAAAGAGGCGGGCGGCCG
CTGGAACAGCATCGGCATCTCCATGGTTTATGTTTCTGAAACAGCATCGCTGACTTTGCTGGAAACACTGGTCCATCTGC
ACTCAGCACACATCCTGGACGCGTTCACACTTCTGCGCATCGATGTTCCCGATGCGCTGATTCAGACCGCCGATATCACT
GAGTTGCCTGCAAACTGGGCCGATGAAGACGCACCGGCAGAACTGGCTGACTATGGAGACGCCTGGTGCTCTGCCTGCGA
TGCGGTTGCGTTGCGTGTTCCCAGTGCCCTCTCCCCTGTAGAATATAACTATCTGTTAAATCCTGAACACCCCGATTTCT
TCCGTATAGTTCAACAGGCTGAGGCTATTCCGTTCCGGTTTGATCGTCGGCTGAAACCGGATCGTAAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT154945 WP_000554758.1 NZ_CP051711:3976211-3976504 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT154945 NZ_CP068443:241397-241840 [Pantoea agglomerans]
ATGAAAACATTCAGCTTTTCTGCGAATAAAACCAGGGCGCCACGCTTATGGCAGGCCGCAGGCCTGAACAACGCTGATGG
TGTCGCCCTGCTGGGCCAGATTAATGAAGGCCTTGATGGCAACGTAGCTCACCTGATCGTCAACTGGGCGAAGATTACGC
AAAACGATCTGCGCAAAATGTCCGGTATTCCCTCCACGACGTTCAGCCGGAGCGTGAAAGCGAAATTCAGCGCTGAACAA
AGTGAACGACTGGTACGAATTATCCGTGTCATTGATCGCGCAGTTGAACTGTTCGAAGGTGATAAGGATGAAGCGCAAAA
ATGGTTGAATGAGCCTAACAGGGCGCTGAGCTGGAAAATGCCCGCAGAACTGATGGCGTCTGAAACCGGGGCATACGAGG
TCATGAAATTAATCACGCGCCTGGAGCACGGAGTTTACTCTTGA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3