Detailed information of TA system TA154936

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2995369..2995971
• Replicon: chromosome
• Accession: NZ_CP051711
• Organism: Escherichia coli strain SCU-123

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: HHJ36_RS14345
• Protein ID: WP_000897302.1
• Coordinates: 2995369..2995680 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HHJ36_RS14350
• Protein ID: WP_000356397.1
• Coordinates: 2995681..2995971 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ36_RS14320	2991283..2991882	+	600	WP_001314324.1	glucose-1-phosphatase	-
HHJ36_RS14325	2991876..2992748	+	873	WP_000920762.1	virulence factor BrkB family protein	-
HHJ36_RS14330	2992745..2993182	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HHJ36_RS14335	2993227..2994168	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HHJ36_RS14340	2994232..2995140	-	909	WP_001331553.1	alpha/beta hydrolase	-
HHJ36_RS14345	2995369..2995680	+	312	WP_000897302.1	hypothetical protein	Toxin
HHJ36_RS14350	2995681..2995971	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HHJ36_RS14355	2996330..2996608	+	279	WP_001296612.1	hypothetical protein	-
HHJ36_RS14360	2997004..2997222	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
HHJ36_RS14365	2997438..2998367	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
HHJ36_RS14370	2998364..2998999	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HHJ36_RS14375	2998996..2999898	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T154936 WP_000897302.1 NZ_CP051711:2995369-2995680 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T154936 NZ_CP068437:1414716-1415141 [Shewanella sp. KX20019]
GTGGTCGCCCAGCAGCATAGTTTTCACCTTTCCAGTTCTTTCGACCAACGATTCTCGTTGGTCGTTGTTGCGAGTCTCTG
CTTCATCTCTTTTATGGTTTGGCCTTATGGTGAATCATTCAGCTATCTTTTTTGTAAATCGCTAATCTTTATCCTGAGTA
TTAGCTATTTCAGTTGGCAGTTTTGGCAACTAAAACACTGGTCTTGTCAGTTTATATTATCTGCAGAAGGTAATGGTCGA
CTTAACCAAAAGCAGGGCTTTGTGATCAAAGGTAAACCAATGATCACTCCGTTTGCGTTGATGTTTGATATCCAGTGCGA
GACTGAAGTCAAAAGACTGGTGGTGTGGGCTGATATGTTAGACGACACAAGCTATCGCCATTTGTGTCGTCTGTTGTTGC
AATCGAATCAAAGGCGGCTTAACTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT154936 WP_000356397.1 NZ_CP051711:2995681-2995971 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT154936 NZ_CP068437:1414496-1414735 [Shewanella sp. KX20019]
ATGAAAATAGCAAGAGTTAGATGGGCTTGTCGTAGAGGCATGTTAGAGCTAGATGTGTTGTTTCAACCTTTTGTTGAGCT
GCACTATGAAGCTATGTCTAATGCTGATAAAAATATTTTTGTCAGGTTGTTAGAGTCTGAAGACCCAGAGCTTTTTGCGT
GGTTTATGGGACATGAGGTCTGCAAAGACCCTGAGTTTGCAGAGATGATCGTTAGAGTTCGTGGTCGCCCAGCAGCATAG

Structures

Toxin

• AlphaFold DB: S1P416