Detailed information of TA system TA154932

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 827496..827998
• Replicon: chromosome
• Accession: NZ_CP051711
• Organism: Escherichia coli strain SCU-123

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A0H2Z0A6
• Locus tag: HHJ36_RS03935
• Protein ID: WP_000767826.1
• Coordinates: 827496..827750 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: HHJ36_RS03940
• Protein ID: WP_071686836.1
• Coordinates: 827747..827998 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ36_RS03915	823989..825347	-	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
HHJ36_RS03920	825337..825399	-	63	WP_010723108.1	membrane protein YoeI	-
HHJ36_RS03925	825614..826543	-	930	WP_000803363.1	LysR family transcriptional regulator	-
HHJ36_RS03930	826589..827413	-	825	WP_000754737.1	SDR family oxidoreductase	-
HHJ36_RS03935	827496..827750	-	255	WP_000767826.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
HHJ36_RS03940	827747..827998	-	252	WP_071686836.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
HHJ36_RS03945	828281..828331	+	51	WP_001364200.1	his operon leader peptide	-
HHJ36_RS03950	828477..829376	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
HHJ36_RS03955	829382..830686	+	1305	WP_001350692.1	histidinol dehydrogenase	-
HHJ36_RS03960	830683..831753	+	1071	WP_000108997.1	histidinol-phosphate transaminase	-
HHJ36_RS03965	831753..832820	+	1068	WP_000080128.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10195.64 Da
• Isoelectric Point: 8.0353

>T154932 WP_000767826.1 NZ_CP051711:c827750-827496 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRIIEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T154932 NZ_CP068435:c1093329-1092940 [Cupriavidus necator]
ATGAGCGTGCTCGTCGACACCTCTGTTTGGGTAGACCATTTCAAGAGCCGCAATGACGCACTGGTCAGCTTGCTCGTGTC
GGATCAAGCGCTGACCCACCCCATGGTCGTGGTTGAACTAGCTTGCGGGACGCCCCCGGCACCGCGTGCCCGGACACTCG
GCGACATCGGCCTGTTGCAGCCTGCAAGGCGGGCGAGCCTGGCAGAAGTGATGGCTTTCATCGAGCAAGAGAATCTGTAT
GGACTGGGCTGCGGCCTGGTGGATGTCTCATTGCTGGCTTCCACTTTGATTACGCCAGGCGCACGGCTATGGACACTGGA
CCAGCGTCTCAACGACTTGTCAGCGCGTTTTGGTGTGGCGCATCGACCCAGGCTTCACCAGGTCACCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9333.62 Da
• Isoelectric Point: 4.7954

>AT154932 WP_071686836.1 NZ_CP051711:c827998-827747 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEPCVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT154932 NZ_CP068435:c1093532-1093326 [Cupriavidus necator]
ATGCGCACGACCGTCACGATCGACGACGCCCTGTATGAACAAGCACTAGAGGTGGCTGACCCTGGCATGGACAAATCTGA
CCTTTTTCGTGAGGCAATCAAGACTTTCGTTCGGGTTCAAGCGGCCAAGCGTCTCGCGGCACTGGGTGGCGCCGCCCCCA
ACATGGAAGACGCGCCACGTCGCCGTATGGAACCTGAAGCAAAATGA

Structures

Toxin

• AlphaFold DB: A0A0H2Z0A6