Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 204085..204647 Replicon chromosome
Accession NZ_CP051711
Organism Escherichia coli strain SCU-123

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag HHJ36_RS00970 Protein ID WP_000605675.1
Coordinates 204369..204647 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag HHJ36_RS00965 Protein ID WP_000781370.1
Coordinates 204085..204369 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ36_RS00950 199266..202313 + 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -
HHJ36_RS00955 202326..203210 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
HHJ36_RS00960 203203..203856 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
HHJ36_RS00965 204085..204369 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
HHJ36_RS00970 204369..204647 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HHJ36_RS00975 204833..205843 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
HHJ36_RS00980 205977..207674 - 1698 WP_000433462.1 malate dehydrogenase -
HHJ36_RS00985 207830..207967 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
HHJ36_RS00990 208069..208284 - 216 WP_000495766.1 biofilm-dependent modulation protein -
HHJ36_RS00995 208629..209060 + 432 WP_000152310.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T154921 WP_000605675.1 NZ_CP051711:c204647-204369 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T154921 NZ_CP068394:4919410-4919697 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTAAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT154921 WP_000781370.1 NZ_CP051711:c204369-204085 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT154921 NZ_CP068394:4919684-4919986 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
CAAATTCGACGAGGATATTGATTACAGCGACATCCCATCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References