Detailed information of TA system TA154914

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4534777..4535379
• Replicon: chromosome
• Accession: NZ_CP051706
• Organism: Escherichia coli strain SCU-124

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3L4YH82
• Locus tag: HHJ37_RS21740
• Protein ID: WP_000897303.1
• Coordinates: 4535068..4535379 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HHJ37_RS21735
• Protein ID: WP_000356397.1
• Coordinates: 4534777..4535067 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ37_RS21705	4529934..4530863	+	930	WP_001523575.1	formate dehydrogenase accessory protein FdhE	-
HHJ37_RS21710	4531193..4531435	-	243	WP_001523574.1	ribbon-helix-helix domain-containing protein	-
HHJ37_RS21715	4531725..4532573	+	849	WP_001038651.1	hypothetical protein	-
HHJ37_RS21720	4532889..4533338	+	450	WP_001523573.1	hypothetical protein	-
HHJ37_RS21725	4533523..4533741	-	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
HHJ37_RS21730	4534140..4534418	-	279	WP_001296612.1	hypothetical protein	-
HHJ37_RS21735	4534777..4535067	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HHJ37_RS21740	4535068..4535379	-	312	WP_000897303.1	hypothetical protein	Toxin
HHJ37_RS21745	4535608..4536516	+	909	WP_001385591.1	alpha/beta hydrolase	-
HHJ37_RS21750	4536580..4537521	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HHJ37_RS21755	4537566..4538003	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HHJ37_RS21760	4538000..4538872	-	873	WP_000920764.1	virulence factor BrkB family protein	-
HHJ37_RS21765	4538866..4539465	-	600	WP_001296610.1	glucose-1-phosphatase	-
HHJ37_RS21770	4539564..4540349	-	786	WP_001523571.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12302.33 Da
• Isoelectric Point: 10.0233

>T154914 WP_000897303.1 NZ_CP051706:c4535379-4535068 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGRLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T154914 NZ_CP068394:c3192221-3191814 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT154914 WP_000356397.1 NZ_CP051706:c4535067-4534777 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT154914 NZ_CP068394:c3192468-3192202 [Escherichia coli]
ATGGACATTAATAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A3L4YH82