Detailed information of TA system TA154888

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3057879..3058481
• Replicon: chromosome
• Accession: NZ_CP051700
• Organism: Escherichia coli strain SCU-125

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: HHJ38_RS15080
• Protein ID: WP_000897302.1
• Coordinates: 3058170..3058481 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HHJ38_RS15075
• Protein ID: WP_000356397.1
• Coordinates: 3057879..3058169 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ38_RS15050	3053944..3054846	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HHJ38_RS15055	3054843..3055478	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HHJ38_RS15060	3055475..3056404	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
HHJ38_RS15065	3056628..3056846	-	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
HHJ38_RS15070	3057242..3057520	-	279	WP_001296612.1	hypothetical protein	-
HHJ38_RS15075	3057879..3058169	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HHJ38_RS15080	3058170..3058481	-	312	WP_000897302.1	hypothetical protein	Toxin
HHJ38_RS15085	3058710..3059618	+	909	WP_001331553.1	alpha/beta hydrolase	-
HHJ38_RS15090	3059682..3060623	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HHJ38_RS15095	3060668..3061105	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HHJ38_RS15100	3061102..3061974	-	873	WP_000920762.1	virulence factor BrkB family protein	-
HHJ38_RS15105	3061968..3062567	-	600	WP_001314324.1	glucose-1-phosphatase	-
HHJ38_RS15110	3062666..3063451	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T154888 WP_000897302.1 NZ_CP051700:c3058481-3058170 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T154888 NZ_CP068386:c4224188-4223775 [Salmonella enterica subsp. enterica serovar Gallinarum/Pullorum]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT154888 WP_000356397.1 NZ_CP051700:c3058169-3057879 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT154888 NZ_CP068386:c4224435-4224169 [Salmonella enterica subsp. enterica serovar Gallinarum/Pullorum]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGACGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: S1P416