Detailed information of TA system TA154881

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2020075..2020769
• Replicon: chromosome
• Accession: NZ_CP051700
• Organism: Escherichia coli strain SCU-125

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: HHJ38_RS10165
• Protein ID: WP_001263500.1
• Coordinates: 2020075..2020473 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: HHJ38_RS10170
• Protein ID: WP_000554758.1
• Coordinates: 2020476..2020769 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ38_RS10140	2015440..2015898	-	459	WP_001291991.1	xanthine phosphoribosyltransferase	-
HHJ38_RS10145	2016159..2017616	+	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
HHJ38_RS10150	2017673..2018287	-	615	WP_000602123.1	peptide chain release factor H	-
HHJ38_RS10155	2018284..2019435	-	1152	WP_001335538.1	RNA ligase RtcB family protein	-
HHJ38_RS10160	2019613..2020065	-	453	WP_071778924.1	GNAT family N-acetyltransferase	-
HHJ38_RS10165	2020075..2020473	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HHJ38_RS10170	2020476..2020769	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HHJ38_RS10175	2020821..2021876	-	1056	WP_001226168.1	DNA polymerase IV	-
HHJ38_RS10180	2021947..2022732	-	786	WP_061301231.1	putative lateral flagellar export/assembly protein LafU	-
HHJ38_RS10185	2022677..2024416	+	1740	WP_021523756.1	flagellar biosynthesis protein FlhA	-
HHJ38_RS10190	2024495..2024839	+	345	WP_125093574.1	hypothetical protein	-
HHJ38_RS10195	2024834..2025331	-	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T154881 WP_001263500.1 NZ_CP051700:c2020473-2020075 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T154881 NZ_CP068386:992267-992575 [Salmonella enterica subsp. enterica serovar Gallinarum/Pullorum]
ATGCAATACATGGTTTATCACAATAAAGGAAACAGCAAGGCTTATCCTTATTTACTTGATGTGCAAAGCGATATTATTGA
TGAACTGCATACTCGCATGGTTATCCCATTGTTTCCTGTGAGCAGATTGGTAAATAACCCAGTTAAGCGCCTCACGCCCA
CTCTGAATGTGGAAGGTAATGACTATCTGGTGATGACTCACGAAATGGCGAGTATCAGGCTTTCGCAGATTGGTGATGAG
GTGATGGACGTTCGTTCCCACAGGCAAACCATCAAAAATGCTCTCGATTTTATCTTTGACGGGTTCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT154881 WP_000554758.1 NZ_CP051700:c2020769-2020476 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT154881 NZ_CP068386:992025-992264 [Salmonella enterica subsp. enterica serovar Gallinarum/Pullorum]
ATGCCAGGTATGAGTGTACGACATAAAAAAACAGTCAGTGTCACGCTGGAACCTGCATTGCTTCAGCAAGCCCGTGATGC
AGGTATTAATCTGTCAGCGGTGCTTACCGCCGCGCTGAAAGAGGAAATCAGTGCCACAGGCGTTGAACGCTGGAAAGCAG
AAAATAGAGCGGGTTTGCAGGAGCTCAATCGTATTACTGATGAGCATGGCCTTTTATCGGACGATTACAGGACGTTTTAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3