Detailed information of TA system TA154863

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4555893..4556495
• Replicon: chromosome
• Accession: NZ_CP051698
• Organism: Escherichia coli strain SCU-152

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: HHJ40_RS21630
• Protein ID: WP_000897305.1
• Coordinates: 4556184..4556495 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HHJ40_RS21625
• Protein ID: WP_087906193.1
• Coordinates: 4555893..4556183 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ40_RS21600	4551819..4552721	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HHJ40_RS21605	4552718..4553353	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HHJ40_RS21610	4553350..4554279	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
HHJ40_RS21615	4554609..4554851	-	243	WP_001086388.1	hypothetical protein	-
HHJ40_RS21620	4555070..4555288	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
HHJ40_RS21625	4555893..4556183	-	291	WP_087906193.1	helix-turn-helix domain-containing protein	Antitoxin
HHJ40_RS21630	4556184..4556495	-	312	WP_000897305.1	hypothetical protein	Toxin
HHJ40_RS21635	4556724..4557632	+	909	WP_001162704.1	alpha/beta hydrolase	-
HHJ40_RS21640	4557696..4558637	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HHJ40_RS21645	4558682..4559119	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HHJ40_RS21650	4559116..4559988	-	873	WP_000920762.1	virulence factor BrkB family protein	-
HHJ40_RS21655	4559982..4560581	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T154863 WP_000897305.1 NZ_CP051698:c4556495-4556184 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T154863 NZ_CP068287:803608-804018 [Enterobacter hormaechei]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCACAGTGGATGTCCTTACTGCTCCACGGTCTGGTCGCGGC
AATGGTGTTATTGGTGCCGTGGCCGCTAAGCTATACCCCTCTGTGGCTGCTTTTGCTGTCGTTTGTAGTGTTTGACAGCG
TGCGCAGCCAGCGTCGAATCAACGCCCGTCAGGGTGAAATTAAGCTGCTGATGGATTCTCGCCTGCGCTGGCAGGGAAAA
GAGTGGGAAATGATAGGGACGCCGTGGATGCTCAACACCGGGATGATGCTACGGCTGCGTCGGGTCGAGGACAATCGTCG
CCAGCACCTGTGGCTGGCAGCAGACAGTATGGATCCTGCGGAGTGGCGAGATCTGCGCCGGTTGATCGTGCAACAGCCGC
CGCAGGAGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10894.51 Da
• Isoelectric Point: 10.9288

>AT154863 WP_087906193.1 NZ_CP051698:c4556183-4555893 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVYAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT154863 NZ_CP068287:803361-803627 [Enterobacter hormaechei]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAGTATGAGTACGACAGCTTAAGCGATGACGATAAGCGCCTGTTTGTCCGTCTGCTTGAGTCCGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7