Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 659197..659996 Replicon chromosome
Accession NZ_CP051698
Organism Escherichia coli strain SCU-152

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag HHJ40_RS03170 Protein ID WP_000347267.1
Coordinates 659197..659661 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HHJ40_RS03175 Protein ID WP_001307405.1
Coordinates 659661..659996 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ40_RS03140 654198..654632 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
HHJ40_RS03145 654650..655528 - 879 WP_001355782.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
HHJ40_RS03150 655518..656297 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
HHJ40_RS03155 656308..656781 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HHJ40_RS03160 656804..658084 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
HHJ40_RS03165 658333..659142 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HHJ40_RS03170 659197..659661 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HHJ40_RS03175 659661..659996 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HHJ40_RS03180 660145..661716 - 1572 WP_001273741.1 galactarate dehydratase -
HHJ40_RS03185 662091..663425 + 1335 WP_000599648.1 galactarate/glucarate/glycerate transporter GarP -
HHJ40_RS03190 663441..664211 + 771 WP_001559981.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T154842 WP_000347267.1 NZ_CP051698:c659661-659197 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T154842 NZ_CP068283:c2960945-2960610 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT154842 WP_001307405.1 NZ_CP051698:c659996-659661 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT154842 NZ_CP068283:c2961193-2960945 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References