Detailed information of TA system TA154837

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 66047..66300
• Replicon: plasmid pSCU-313-2
• Accession: NZ_CP051696
• Organism: Escherichia coli strain SCU-313

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A148HBD8
• Locus tag: HHJ46_RS25425
• Protein ID: WP_001336447.1
• Coordinates: 66151..66300 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	66047..66103 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ46_RS25395	61960..62706	+	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-
HHJ46_RS25400	62761..63318	+	558	WP_000139329.1	fertility inhibition protein FinO	-
HHJ46_RS25405	63470..63673	+	204	WP_001336517.1	hypothetical protein	-
HHJ46_RS25410	64415..64876	+	462	WP_000760080.1	thermonuclease family protein	-
HHJ46_RS25415	64979..65362	+	384	WP_001109264.1	hypothetical protein	-
HHJ46_RS25420	65515..65952	+	438	WP_000872609.1	hypothetical protein	-
-	66047..66103	-	57	NuclAT_1	-	Antitoxin
-	66047..66103	-	57	NuclAT_1	-	Antitoxin
-	66047..66103	-	57	NuclAT_1	-	Antitoxin
-	66047..66103	-	57	NuclAT_1	-	Antitoxin
HHJ46_RS25425	66151..66300	+	150	WP_001336447.1	type I toxin-antitoxin system Hok family toxin	Toxin
HHJ46_RS25430	66585..66842	+	258	WP_000084404.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..67142	67142

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5572.69 Da
• Isoelectric Point: 8.7678

>T154837 WP_001336447.1 NZ_CP051696:66151-66300 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T154837 NZ_CP068283:2145289-2145663 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Nucleotide

• Length: 57 bp

>AT154837 NZ_CP051696:c66103-66047 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A148HBD8

Antitoxin