Detailed information of TA system TA154821

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3957043..3957693
• Replicon: chromosome
• Accession: NZ_CP051694
• Organism: Escherichia coli strain SCU-313

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HHJ46_RS19145
• Protein ID: WP_000263532.1
• Coordinates: 3957352..3957693 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: U9YXQ7
• Locus tag: HHJ46_RS19140
• Protein ID: WP_000212552.1
• Coordinates: 3957043..3957342 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ46_RS19115	3952491..3952802	-	312	WP_000415004.1	hypothetical protein	-
HHJ46_RS19120	3952807..3953199	-	393	WP_000285322.1	flagellar export chaperone FliS	-
HHJ46_RS19125	3953222..3954538	-	1317	WP_000609678.1	flagellar filament capping protein FliD	-
HHJ46_RS19130	3954743..3955657	-	915	WP_000949083.1	lateral flagellin LafA	-
HHJ46_RS19135	3956143..3956985	+	843	WP_000022366.1	winged helix-turn-helix domain-containing protein	-
HHJ46_RS19140	3957043..3957342	-	300	WP_000212552.1	helix-turn-helix transcriptional regulator	Antitoxin
HHJ46_RS19145	3957352..3957693	-	342	WP_000263532.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HHJ46_RS19150	3957769..3958746	-	978	WP_001195938.1	hypothetical protein	-
HHJ46_RS19155	3958763..3959692	-	930	WP_001266799.1	flagellar hook-associated protein FlgL	-
HHJ46_RS19160	3959707..3961083	-	1377	WP_000367245.1	flagellar hook-associated protein FlgK	-
HHJ46_RS19165	3961259..3961558	-	300	WP_000867281.1	rod-binding protein	-
HHJ46_RS19170	3961558..3962658	-	1101	WP_001181876.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3946952..3957696	10744

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13031.86 Da
• Isoelectric Point: 6.4677

>T154821 WP_000263532.1 NZ_CP051694:c3957693-3957352 [Escherichia coli]
MWDVETTETFDNWFDAQTVALKEDLLAAMLILAEYGPQLGRPFADTVNDSQFSNMKELRVQHQGNPVRAFFAFDPARRGI
VLCAGDKTGLNEKKFYRDMIKLADSEYRKHLKK

Nucleotide

• Length: 342 bp

>T154821 NZ_CP068281:4524195-4524659 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11004.86 Da
• Isoelectric Point: 9.7665

>AT154821 WP_000212552.1 NZ_CP051694:c3957342-3957043 [Escherichia coli]
MATLKELMAKQSPESQVRIAAKVEEMRQTIALHQLREELNISQTEMAAAMGIKQPTLAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPNGKRIAFQI

Nucleotide

• Length: 300 bp

>AT154821 NZ_CP068281:4523860-4524195 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: U9YXQ7