Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2630385..2630947 Replicon chromosome
Accession NZ_CP051694
Organism Escherichia coli strain SCU-313

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag HHJ46_RS12650 Protein ID WP_000605676.1
Coordinates 2630385..2630663 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag HHJ46_RS12655 Protein ID WP_000781370.1
Coordinates 2630663..2630947 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ46_RS12625 2625971..2626402 - 432 WP_000152305.1 peroxiredoxin OsmC -
HHJ46_RS12630 2626747..2626962 + 216 WP_000495766.1 biofilm-dependent modulation protein -
HHJ46_RS12635 2627064..2627201 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
HHJ46_RS12640 2627358..2629055 + 1698 WP_000433464.1 malate dehydrogenase -
HHJ46_RS12645 2629189..2630199 + 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
HHJ46_RS12650 2630385..2630663 + 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HHJ46_RS12655 2630663..2630947 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
HHJ46_RS12660 2631175..2631828 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
HHJ46_RS12665 2631821..2632705 - 885 WP_001240565.1 formate dehydrogenase N subunit beta -
HHJ46_RS12670 2632718..2635765 - 3048 WP_012602782.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T154816 WP_000605676.1 NZ_CP051694:2630385-2630663 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T154816 NZ_CP068281:4016077-4016406 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT154816 WP_000781370.1 NZ_CP051694:2630663-2630947 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT154816 NZ_CP068281:4015739-4016056 [Escherichia coli]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References