Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 635909..636708 Replicon chromosome
Accession NZ_CP051694
Organism Escherichia coli strain SCU-313

Toxin (Protein)


Gene name yhaV Uniprot ID B7NDB6
Locus tag HHJ46_RS03045 Protein ID WP_000347269.1
Coordinates 635909..636373 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID V0YUB5
Locus tag HHJ46_RS03050 Protein ID WP_001309780.1
Coordinates 636373..636708 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ46_RS03015 630910..631344 - 435 WP_000948818.1 PTS sugar transporter subunit IIA -
HHJ46_RS03020 631362..632240 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
HHJ46_RS03025 632230..633009 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
HHJ46_RS03030 633020..633493 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HHJ46_RS03035 633516..634796 - 1281 WP_000681943.1 tagatose-bisphosphate aldolase subunit KbaZ -
HHJ46_RS03040 635045..635854 + 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
HHJ46_RS03045 635909..636373 - 465 WP_000347269.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HHJ46_RS03050 636373..636708 - 336 WP_001309780.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HHJ46_RS03055 636857..638428 - 1572 WP_169062190.1 galactarate dehydratase -
HHJ46_RS03060 638803..640137 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
HHJ46_RS03065 640153..640923 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T154800 WP_000347269.1 NZ_CP051694:c636373-635909 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTREAEETH

Download         Length: 465 bp

>T154800 NZ_CP068281:c1454724-1454446 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12345.94 Da        Isoelectric Point: 4.8616

>AT154800 WP_001309780.1 NZ_CP051694:c636708-636373 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNT
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT154800 NZ_CP068281:c1454987-1454727 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829IY86

Antitoxin

Source ID Structure
AlphaFold DB V0YUB5

References