Detailed information of TA system TA154795

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 9941..10194
• Replicon: plasmid pSCU-387-2
• Accession: NZ_CP051690
• Organism: Escherichia coli strain SCU-387

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: HHJ48_RS26590
• Protein ID: WP_001312851.1
• Coordinates: 9941..10090 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	10135..10194 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ48_RS26560	5970..6371	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
HHJ48_RS26565	6304..6561	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
HHJ48_RS26570	6654..7307	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
HHJ48_RS26575	8230..9047	-	818	Protein_12	incFII family plasmid replication initiator RepA	-
HHJ48_RS26580	9089..9163	-	75	WP_001365705.1	RepA leader peptide Tap	-
HHJ48_RS26585	9408..9656	-	249	WP_000083839.1	replication regulatory protein RepA	-
HHJ48_RS26590	9941..10090	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	10135..10194	+	60	NuclAT_1	-	Antitoxin
-	10135..10194	+	60	NuclAT_1	-	Antitoxin
-	10135..10194	+	60	NuclAT_1	-	Antitoxin
-	10135..10194	+	60	NuclAT_1	-	Antitoxin
HHJ48_RS26595	10337..10810	-	474	WP_016240489.1	hypothetical protein	-
HHJ48_RS26600	10965..11555	-	591	WP_033807967.1	DUF2726 domain-containing protein	-
HHJ48_RS26605	11593..11802	-	210	WP_001298565.1	hemolysin expression modulator Hha	-
HHJ48_RS26610	11848..12309	-	462	WP_001233850.1	thermonuclease family protein	-
HHJ48_RS26615	12555..12767	-	213	WP_001301409.1	ANR family transcriptional regulator	-
HHJ48_RS26620	12899..13459	-	561	WP_033807966.1	fertility inhibition protein FinO	-
HHJ48_RS26625	13514..14260	-	747	WP_000205725.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaTEM-1B / mph(A)	-	1..39292	39292

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T154795 WP_001312851.1 NZ_CP051690:c10090-9941 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T154795 NZ_CP068281:c276427-276320 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT154795 NZ_CP051690:10135-10194 [Escherichia coli]
AATGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTTGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin