Detailed information of TA system TA154791

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 393..663
• Replicon: plasmid pSCU-387-2
• Accession: NZ_CP051690
• Organism: Escherichia coli strain SCU-387

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HHJ48_RS26515
• Protein ID: WP_001312861.1
• Coordinates: 505..663 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	393..456 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ48_RS26510	1..268	+	268	Protein_0	plasmid SOS inhibition protein A	-
-	237..461	+	225	NuclAT_0	-	-
-	237..461	+	225	NuclAT_0	-	-
-	237..461	+	225	NuclAT_0	-	-
-	237..461	+	225	NuclAT_0	-	-
-	393..456	-	64	-	-	Antitoxin
HHJ48_RS26515	505..663	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
HHJ48_RS26520	901..1278	-	378	Protein_2	hypothetical protein	-
HHJ48_RS26530	1578..1820	+	243	WP_169062313.1	hypothetical protein	-
HHJ48_RS26535	1854..2558	-	705	WP_001067858.1	IS6-like element IS26 family transposase	-
HHJ48_RS26540	2624..3055	+	432	Protein_5	recombinase family protein	-
HHJ48_RS26545	3238..4098	+	861	WP_000027057.1	class A broad-spectrum beta-lactamase TEM-1	-
HHJ48_RS26550	4248..4346	-	99	Protein_7	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaTEM-1B / mph(A)	-	1..39292	39292

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T154791 WP_001312861.1 NZ_CP051690:505-663 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T154791 NZ_CP068279:4426580-4427044 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Nucleotide

• Length: 64 bp

>AT154791 NZ_CP051690:c456-393 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin