Detailed information of TA system TA154785

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 74817..75070
• Replicon: plasmid pSCU-387-1
• Accession: NZ_CP051689
• Organism: Escherichia coli strain SCU-387

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: HHJ48_RS26105
• Protein ID: WP_021515405.1
• Coordinates: 74817..74966 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	75011..75070 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ48_RS26075	70184..70447	-	264	Protein_52	type II toxin-antitoxin system RelE/ParE family toxin	-
HHJ48_RS26080	70437..70700	-	264	WP_001089474.1	hypothetical protein	-
HHJ48_RS26895	70807..71067	-	261	WP_097403166.1	hypothetical protein	-
HHJ48_RS26085	71768..72625	-	858	WP_123004306.1	incFII family plasmid replication initiator RepA	-
HHJ48_RS26090	72618..72692	-	75	WP_001367777.1	RepA leader peptide Tap	-
HHJ48_RS26095	72926..73183	-	258	WP_001620838.1	replication regulatory protein RepA	-
HHJ48_RS26100	73485..74183	-	699	WP_115766398.1	acid phosphatase AphA	-
HHJ48_RS26900	74369..74662	-	294	WP_211202395.1	hypothetical protein	-
HHJ48_RS26105	74817..74966	-	150	WP_021515405.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	75011..75070	+	60	NuclAT_1	-	Antitoxin
-	75011..75070	+	60	NuclAT_1	-	Antitoxin
-	75011..75070	+	60	NuclAT_1	-	Antitoxin
-	75011..75070	+	60	NuclAT_1	-	Antitoxin
HHJ48_RS26110	75245..75553	-	309	WP_001620832.1	hypothetical protein	-
HHJ48_RS26115	75550..76236	-	687	WP_001620830.1	DUF2726 domain-containing protein	-
HHJ48_RS26120	76274..76483	-	210	WP_097424846.1	hemolysin expression modulator Hha	-
HHJ48_RS26125	76529..76990	-	462	WP_052983101.1	thermonuclease family protein	-
HHJ48_RS26130	77235..77447	-	213	WP_097424844.1	ANR family transcriptional regulator	-
HHJ48_RS26135	77582..78142	-	561	WP_001620825.1	fertility inhibition protein FinO	-
HHJ48_RS26140	78197..78943	-	747	WP_000205725.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	iroB / iroC / iroD / iroE / iroN / senB	1..139635	139635
	-	inside	IScluster/Tn	-	-	65452..70103	4651

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5604.75 Da
• Isoelectric Point: 8.7678

>T154785 WP_021515405.1 NZ_CP051689:c74966-74817 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVMQVTLAYEARK

Nucleotide

• Length: 150 bp

>T154785 NZ_CP068279:3905591-3906664 [Escherichia coli]
ATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCAAATATCGAGACTGAATCCAGACAATTCATTGAAAATAA
AAACTATTCAATCCAATCAATTGGTCCTATGCCAGGGTCAAGGGCTGGGCTTCGGGTCGTATTTACCAGACCAGGGGTTA
ACTTGGCAACTGTGGACATTTTTTATAACGGGGACGGTTCGACTACAATTCAATATCTCACTGGAGCCAATCGTTCTCTG
GGCCAAGAGTTAGCGGATCATCTTTTTGAAACCATCAATCCTGCTGAATTTGAGCAGGTAAATATGGTACTGCAAGGATT
TGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACG
CTCATACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAG
CTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGCAGCCCTGCTTGATTTACAGGGTTT
GGAGAAAGTGCTAATCCGCCAGGAGGATGGTAAAGCTAATATTGTTCAACAGGAGGTTGCCCGCACTTACTTGCAGACTG
TAATGGCCGATGCTTACCCGCATCTCCACGTGACTGCCGAAAAATTGCTCGTTTCAGGGCTATGTGTTAAACTCGCCGCC
CCTGATTTGCCTGACTACTGTATGTTACTTTATCCTGAACTACGCACCATTGAAGGTGTCTTAAAAAGTAAGATGAGTGG
GTTAGGCATGCCAGTACAGCAGCCGGCAGGTTTTGGAACTTACTTTGATAAACCTGCTGCTCATTACATTCTGAAACCGC
AATTTGCAGCTACTCTTAGACCGGAACAGATTAACATCATCAGCACAGCCTATACTTTTTTTAATGTGGAACGTCATTCT
CTGTTCCACATGGAAACTGTGGTCGATGCCAGCCGTATGATTTCTGATATGGCCCGGTTGATGGGTAAAGCCACTAGAGC
GTGGGGAATAATCAAGGACTTATATATTGTTTGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT154785 NZ_CP051689:75011-75070 [Escherichia coli]
TAAGTACATCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin