Detailed information of TA system TA154783

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5301379..5301981
• Replicon: chromosome
• Accession: NZ_CP051688
• Organism: Escherichia coli strain SCU-387

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: HHJ48_RS25655
• Protein ID: WP_000897302.1
• Coordinates: 5301379..5301690 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HHJ48_RS25660
• Protein ID: WP_000356397.1
• Coordinates: 5301691..5301981 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ48_RS25625	5296409..5297194	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
HHJ48_RS25630	5297293..5297892	+	600	WP_001314324.1	glucose-1-phosphatase	-
HHJ48_RS25635	5297886..5298758	+	873	WP_000920762.1	virulence factor BrkB family protein	-
HHJ48_RS25640	5298755..5299192	+	438	WP_000560978.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HHJ48_RS25645	5299237..5300178	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HHJ48_RS25650	5300242..5301150	-	909	WP_001331553.1	alpha/beta hydrolase	-
HHJ48_RS25655	5301379..5301690	+	312	WP_000897302.1	hypothetical protein	Toxin
HHJ48_RS25660	5301691..5301981	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HHJ48_RS25665	5302340..5302618	+	279	WP_001296612.1	hypothetical protein	-
HHJ48_RS25670	5303014..5303232	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
HHJ48_RS25675	5303448..5304377	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
HHJ48_RS25680	5304374..5305009	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HHJ48_RS25685	5305006..5305908	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T154783 WP_000897302.1 NZ_CP051688:5301379-5301690 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T154783 NZ_CP068279:c3248341-3248087 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT154783 WP_000356397.1 NZ_CP051688:5301691-5301981 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT154783 NZ_CP068279:c3248589-3248338 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Structures

Toxin

• AlphaFold DB: S1P416