Detailed information of TA system TA154774

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2388068..2388630
• Replicon: chromosome
• Accession: NZ_CP051688
• Organism: Escherichia coli strain SCU-387

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1Q1V8
• Locus tag: HHJ48_RS11670
• Protein ID: WP_000605675.1
• Coordinates: 2388068..2388346 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A8D9UMF3
• Locus tag: HHJ48_RS11675
• Protein ID: WP_021528209.1
• Coordinates: 2388346..2388630 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ48_RS11645	2383654..2384085	-	432	WP_000152305.1	peroxiredoxin OsmC	-
HHJ48_RS11650	2384430..2384645	+	216	WP_000495766.1	biofilm-dependent modulation protein	-
HHJ48_RS11655	2384747..2384884	+	138	WP_000841554.1	stationary-phase-induced ribosome-associated protein	-
HHJ48_RS11660	2385041..2386738	+	1698	WP_000433460.1	malate dehydrogenase	-
HHJ48_RS11665	2386872..2387882	+	1011	WP_000642412.1	alcohol dehydrogenase AdhP	-
HHJ48_RS11670	2388068..2388346	+	279	WP_000605675.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HHJ48_RS11675	2388346..2388630	+	285	WP_021528209.1	HigA family addiction module antidote protein	Antitoxin
HHJ48_RS11680	2388859..2389512	-	654	WP_000045647.1	formate dehydrogenase-N subunit gamma	-
HHJ48_RS11685	2389505..2390389	-	885	WP_001240584.1	formate dehydrogenase N subunit beta	-
HHJ48_RS11690	2390402..2393449	-	3048	WP_011478186.1	formate dehydrogenase-N subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-65)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10537.16 Da
• Isoelectric Point: 7.3206

>T154774 WP_000605675.1 NZ_CP051688:2388068-2388346 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Nucleotide

• Length: 279 bp

>T154774 NZ_CP068279:c1622404-1622186 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10429.14 Da
• Isoelectric Point: 8.8567

>AT154774 WP_021528209.1 NZ_CP051688:2388346-2388630 [Escherichia coli]
MKMVNHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Nucleotide

• Length: 285 bp

>AT154774 NZ_CP068279:c1622804-1622430 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: S1Q1V8