Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1037216..1037910 Replicon chromosome
Accession NZ_CP051688
Organism Escherichia coli strain SCU-387

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag HHJ48_RS04920 Protein ID WP_001263500.1
Coordinates 1037512..1037910 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag HHJ48_RS04915 Protein ID WP_000554758.1
Coordinates 1037216..1037509 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ48_RS04890 1033033..1033500 + 468 WP_000725261.1 flagellar basal body-associated FliL family protein -
HHJ48_RS04895 1033520..1034236 + 717 WP_000938731.1 FliA/WhiG family RNA polymerase sigma factor -
HHJ48_RS04900 1034249..1035112 + 864 WP_001169518.1 flagellar motor stator protein MotA -
HHJ48_RS04905 1035115..1036038 + 924 WP_001532973.1 putative lateral flagellar export/assembly protein LafU -
HHJ48_RS04910 1036109..1037164 + 1056 WP_001226168.1 DNA polymerase IV -
HHJ48_RS04915 1037216..1037509 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
HHJ48_RS04920 1037512..1037910 + 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
HHJ48_RS04925 1037920..1038372 + 453 WP_001059895.1 GNAT family N-acetyltransferase -
HHJ48_RS04930 1038562..1039701 + 1140 WP_000521561.1 RNA ligase RtcB family protein -
HHJ48_RS04935 1039698..1040312 + 615 WP_000602124.1 peptide chain release factor H -
HHJ48_RS04940 1040369..1041826 - 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
HHJ48_RS04945 1042087..1042545 + 459 WP_001291991.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T154762 WP_001263500.1 NZ_CP051688:1037512-1037910 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T154762 NZ_CP068278:c99903-99640 [Corynebacterium aquatimens]
GTGAGAATCATGTCCTACACGGAGTCCCGCGCCAAGTATGCAGACATGCTCACTTCAGTAGTGGATGACTGCGAAGAAGT
CATCATCACCCGCGCAGGCCACGAGCCCGTAGTTGTCGTCTCCCTGGCTGAGTACGAGTCCCTCAAGGAGACCGCCTACC
TGCTCCGCTCGCCCGCAAACGCACGCATGCTGAAAGAACGGATTGCCCGGGTGGAACGGGGGCAAGCGGAAGAGCACGAG
CTCATTGAGGATGACACGTGTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT154762 WP_000554758.1 NZ_CP051688:1037216-1037509 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT154762 NZ_CP068278:c99646-99386 [Corynebacterium aquatimens]
GTGTTAGTCCGTTGGGAGCGGGACGCATGGGAAGAATATTGCCGGAGGCAAATTGAGGATCGGAGGATCCTCAAACGAAT
TAACACCCTGATCACTGACGTATTGCGGAACGGCAATGAGGGTATTGGTAAACCGGAACCGCTGAAGCACGACTTTTCGG
GGTACTGGTCACGCCGCATCAACAGCGAACACCGGCTGATTTACAAGATCGACGACGGCGGCATAACCATCGTTGCTTGC
CGCTACCACTACGGTGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References