Detailed information of TA system TA154762

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1037216..1037910
• Replicon: chromosome
• Accession: NZ_CP051688
• Organism: Escherichia coli strain SCU-387

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: HHJ48_RS04920
• Protein ID: WP_001263500.1
• Coordinates: 1037512..1037910 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: HHJ48_RS04915
• Protein ID: WP_000554758.1
• Coordinates: 1037216..1037509 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ48_RS04890	1033033..1033500	+	468	WP_000725261.1	flagellar basal body-associated FliL family protein	-
HHJ48_RS04895	1033520..1034236	+	717	WP_000938731.1	FliA/WhiG family RNA polymerase sigma factor	-
HHJ48_RS04900	1034249..1035112	+	864	WP_001169518.1	flagellar motor stator protein MotA	-
HHJ48_RS04905	1035115..1036038	+	924	WP_001532973.1	putative lateral flagellar export/assembly protein LafU	-
HHJ48_RS04910	1036109..1037164	+	1056	WP_001226168.1	DNA polymerase IV	-
HHJ48_RS04915	1037216..1037509	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HHJ48_RS04920	1037512..1037910	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HHJ48_RS04925	1037920..1038372	+	453	WP_001059895.1	GNAT family N-acetyltransferase	-
HHJ48_RS04930	1038562..1039701	+	1140	WP_000521561.1	RNA ligase RtcB family protein	-
HHJ48_RS04935	1039698..1040312	+	615	WP_000602124.1	peptide chain release factor H	-
HHJ48_RS04940	1040369..1041826	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
HHJ48_RS04945	1042087..1042545	+	459	WP_001291991.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T154762 WP_001263500.1 NZ_CP051688:1037512-1037910 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T154762 NZ_CP068278:c99903-99640 [Corynebacterium aquatimens]
GTGAGAATCATGTCCTACACGGAGTCCCGCGCCAAGTATGCAGACATGCTCACTTCAGTAGTGGATGACTGCGAAGAAGT
CATCATCACCCGCGCAGGCCACGAGCCCGTAGTTGTCGTCTCCCTGGCTGAGTACGAGTCCCTCAAGGAGACCGCCTACC
TGCTCCGCTCGCCCGCAAACGCACGCATGCTGAAAGAACGGATTGCCCGGGTGGAACGGGGGCAAGCGGAAGAGCACGAG
CTCATTGAGGATGACACGTGTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT154762 WP_000554758.1 NZ_CP051688:1037216-1037509 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT154762 NZ_CP068278:c99646-99386 [Corynebacterium aquatimens]
GTGTTAGTCCGTTGGGAGCGGGACGCATGGGAAGAATATTGCCGGAGGCAAATTGAGGATCGGAGGATCCTCAAACGAAT
TAACACCCTGATCACTGACGTATTGCGGAACGGCAATGAGGGTATTGGTAAACCGGAACCGCTGAAGCACGACTTTTCGG
GGTACTGGTCACGCCGCATCAACAGCGAACACCGGCTGATTTACAAGATCGACGACGGCGGCATAACCATCGTTGCTTGC
CGCTACCACTACGGTGAATAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3