Detailed information of TA system TA154733

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 82072..82311
• Replicon: plasmid pEco1162invT2b
• Accession: NZ_CP051665
• Organism: Escherichia coli strain 1162invT2

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: HH200_RS24995
• Protein ID: WP_023144756.1
• Coordinates: 82072..82206 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	82251..82311 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HH200_RS24960 (78083)	78083..78484	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
HH200_RS24965 (78417)	78417..78674	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
HH200_RS24970 (78767)	78767..79420	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
HH200_RS24975 (80360)	80360..81217	-	858	WP_001617855.1	incFII family plasmid replication initiator RepA	-
HH200_RS24980 (81210)	81210..81284	-	75	WP_031943482.1	RepA leader peptide Tap	-
HH200_RS24985 (81284)	81284..81415	-	132	Protein_99	protein CopA/IncA	-
HH200_RS24990 (81521)	81521..81775	-	255	WP_000083850.1	replication regulatory protein RepA	-
HH200_RS24995 (82072)	82072..82206	-	135	WP_023144756.1	Hok/Gef family protein	Toxin
- (82251)	82251..82311	+	61	NuclAT_2	-	Antitoxin
- (82251)	82251..82311	+	61	NuclAT_2	-	Antitoxin
- (82251)	82251..82311	+	61	NuclAT_2	-	Antitoxin
- (82251)	82251..82311	+	61	NuclAT_2	-	Antitoxin
HH200_RS25000 (82278)	82278..82564	-	287	Protein_102	DUF2726 domain-containing protein	-
HH200_RS25005 (82642)	82642..84255	-	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
HH200_RS25010 (84286)	84286..84636	-	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
HH200_RS25015 (84633)	84633..85058	-	426	WP_000422741.1	transposase	-
HH200_RS25020 (85617)	85617..85829	-	213	WP_013023861.1	hypothetical protein	-
HH200_RS25025 (85960)	85960..86520	-	561	WP_000139341.1	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / aac(3)-IId / blaTEM-1B	senB	1..157396	157396

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T154733 WP_023144756.1 NZ_CP051665:c82206-82072 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T154733 NZ_CP068239:c147615-147334 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT154733 NZ_CP051665:82251-82311 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin