Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 4531971..4532650 Replicon chromosome
Accession NZ_CP051663
Organism Escherichia coli strain 1162invT2

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag HH200_RS22080 Protein ID WP_000057523.1
Coordinates 4531971..4532273 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag HH200_RS22085 Protein ID WP_000806442.1
Coordinates 4532309..4532650 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HH200_RS22055 (4527347) 4527347..4528999 + 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
HH200_RS22060 (4529037) 4529037..4529540 - 504 WP_000667000.1 hypothetical protein -
HH200_RS22065 (4529537) 4529537..4530337 - 801 WP_000439798.1 hypothetical protein -
HH200_RS22070 (4530361) 4530361..4530840 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
HH200_RS22075 (4531044) 4531044..4531838 - 795 WP_000365147.1 TraB/GumN family protein -
HH200_RS22080 (4531971) 4531971..4532273 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HH200_RS22085 (4532309) 4532309..4532650 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
HH200_RS22090 (4532708) 4532708..4535212 - 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
HH200_RS22095 (4535474) 4535474..4536406 + 933 WP_000883041.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T154732 WP_000057523.1 NZ_CP051663:4531971-4532273 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T154732 NZ_CP068238:c5345763-5345485 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGATGCGACGAGACTCGTCAGGTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCCATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCTGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACAGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT154732 WP_000806442.1 NZ_CP051663:4532309-4532650 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT154732 NZ_CP068238:c5345473-5345168 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGACGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAACGTCTCCGCCCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGTTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References