Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2488779..2489506 Replicon chromosome
Accession NZ_CP051663
Organism Escherichia coli strain 1162invT2

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag HH200_RS12255 Protein ID WP_000550189.1
Coordinates 2489192..2489506 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HH200_RS12250 Protein ID WP_000560269.1
Coordinates 2488779..2489195 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HH200_RS12240 (2484139) 2484139..2486490 + 2352 WP_000695432.1 alpha-glucosidase -
HH200_RS12245 (2486716) 2486716..2488734 + 2019 WP_000121413.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
HH200_RS12250 (2488779) 2488779..2489195 - 417 WP_000560269.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
HH200_RS12255 (2489192) 2489192..2489506 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
HH200_RS12260 (2489791) 2489791..2490927 - 1137 WP_000018703.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
HH200_RS12265 (2491012) 2491012..2491515 + 504 WP_001295542.1 M48 family metallopeptidase -
HH200_RS12270 (2491592) 2491592..2492284 + 693 WP_000942551.1 vancomycin high temperature exclusion protein -
HH200_RS12275 (2492363) 2492363..2493349 + 987 WP_001385498.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T154719 WP_000550189.1 NZ_CP051663:c2489506-2489192 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T154719 NZ_CP068237:733392-733883 [Klebsiella quasipneumoniae]
ATGATCTCCGCGCCAGAACCGCTTCATGCCGGGCATATACTTACCCCGTTCTGCTGCGGCGTGGAGTCGATGGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTCACTGGCGCATCCCGTACTTTTGTGTGTTGCGGCAGCGATTCTGACGTAC
TGGCGTATTACTCGCTGGCGTCCAGCGCGGTAACGACCAACACCGCCCCCGGTCGCTTTCGTCGCAATATGCCCGATCCG
ATCCCGGTTGTGGTGCTGGGCCGTCTGGCGGTAGATAAATCACTACACGGGCAGGGCGTTGGGCGGGCGCTGGTTCGCGA
CGCCGGGCTGCGGGTGATTCAGGTGGCGGAAACTATCGGCATTCGCGGGATGCTGGTTCATGCCCTGTCGGATGACGCGC
GGGAGTTTTATCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTATTTAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15023.47 Da        Isoelectric Point: 4.4547

>AT154719 WP_000560269.1 NZ_CP051663:c2489195-2488779 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFVEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT154719 NZ_CP068237:733102-733395 [Klebsiella quasipneumoniae]
ATGCCAGCCGCAAACAGTATGACGATGAAACGCGAAACCTTGAACCTGCGCATCAAGCCCGCCGAGCGCGATCTTATCGA
TCGGGCTGCAAAAGCCAGAGGGAAAAACCGAACTGACTTTGTGCTGGAAGCCGCCCGCGCTGCTGCGGAGGAAGCGCTAA
TCGAGCAACGTATCATCATGGCCGATCCGCAGGCTTATCAGGCATTTCTGACTCGGCTGGATCAGGCTCCTGCGCCAAAC
GATGCGTTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References