Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1243053..1243555 Replicon chromosome
Accession NZ_CP051663
Organism Escherichia coli strain 1162invT2

Toxin (Protein)


Gene name yoeB Uniprot ID E2QNP2
Locus tag HH200_RS06540 Protein ID WP_000767819.1
Coordinates 1243053..1243307 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID E2QNP3
Locus tag HH200_RS06545 Protein ID WP_001259253.1
Coordinates 1243304..1243555 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HH200_RS06515 (1238871) 1238871..1239329 - 459 WP_001531805.1 IS200/IS605-like element IS200C family transposase -
HH200_RS06520 (1239546) 1239546..1240904 - 1359 WP_000019194.1 putrescine/proton symporter PlaP -
HH200_RS06525 (1240894) 1240894..1240956 - 63 WP_010723108.1 membrane protein YoeI -
HH200_RS06530 (1241171) 1241171..1242100 - 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
HH200_RS06535 (1242146) 1242146..1242970 - 825 WP_000754737.1 SDR family oxidoreductase -
HH200_RS06540 (1243053) 1243053..1243307 - 255 WP_000767819.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HH200_RS06545 (1243304) 1243304..1243555 - 252 WP_001259253.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HH200_RS06550 (1243838) 1243838..1243888 + 51 WP_001364200.1 his operon leader peptide -
HH200_RS06555 (1244034) 1244034..1244933 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
HH200_RS06560 (1244939) 1244939..1246243 + 1305 WP_001296211.1 histidinol dehydrogenase -
HH200_RS06565 (1246240) 1246240..1247310 + 1071 WP_000108967.1 histidinol-phosphate transaminase -
HH200_RS06570 (1247310) 1247310..1248377 + 1068 WP_000080057.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1238871..1239329 458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10110.53 Da        Isoelectric Point: 7.2749

>T154716 WP_000767819.1 NZ_CP051663:c1243307-1243053 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T154716 NZ_CP068234:c2922393-2922291 [Leclercia adecarboxylata]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9365.62 Da        Isoelectric Point: 4.6127

>AT154716 WP_001259253.1 NZ_CP051663:c1243555-1243304 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Download         Length: 252 bp

>AT154716 NZ_CP068234:2922289-2922459 [Leclercia adecarboxylata]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAGTCGCCTTGCACTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTCCGCAGCAAAAGTGGCCTG
AAAAAAAGCGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E2QNP2

Antitoxin

Source ID Structure
AlphaFold DB E2QNP3

References