Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 4053966..4054645 Replicon chromosome
Accession NZ_CP051661
Organism Escherichia coli strain 1162T7

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag HH201_RS19620 Protein ID WP_000057523.1
Coordinates 4053966..4054268 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag HH201_RS19625 Protein ID WP_000806442.1
Coordinates 4054304..4054645 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HH201_RS19595 (4049342) 4049342..4050994 + 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
HH201_RS19600 (4051032) 4051032..4051535 - 504 WP_000667000.1 hypothetical protein -
HH201_RS19605 (4051532) 4051532..4052332 - 801 WP_000439798.1 hypothetical protein -
HH201_RS19610 (4052356) 4052356..4052835 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
HH201_RS19615 (4053039) 4053039..4053833 - 795 WP_000365147.1 TraB/GumN family protein -
HH201_RS19620 (4053966) 4053966..4054268 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HH201_RS19625 (4054304) 4054304..4054645 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
HH201_RS19630 (4054703) 4054703..4057207 - 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
HH201_RS19635 (4057469) 4057469..4058401 + 933 WP_000883041.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T154695 WP_000057523.1 NZ_CP051661:4053966-4054268 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T154695 NZ_CP068227:c782478-781177 [Shewanella algae]
ATGCAAAAGCTAATAGCATACATGAATGGTGAACTTGTTGGCACCTTGGCGAAGCATAAGAACGGCGCTCACACTTTTCA
ATATGATAAGGACTGGATAACCAATGCAATAGCTCGCCCACTGTCATTGTCTTTGAGGCTTCAATTACCGACTATCACTT
CCGATGCCGTTATCAACTATTTTGACAACCTCTTACCAGACAGCCCACAAGTCAGAGATAGGATCGTTGCAAGGTACAAA
GCCTCGTCCAAACAACCATTTGATCTCTTGAAAGAAATTGGAAAGGATAGTGTTGGTGCTATTGCACTATTTCCTCCCGA
ACACCCCTATAAAAAAGAGCCACTCAGCTACGAAATATTGGATAACAAGAAACTAGAAGCCGTTCTTTCTGCATACAAGT
CTGATATTCCCCTCGGGATGCTCAAAGAGGAAGAAGACTTTAGGATCTCAGTGGCAGGTGCTCAAGAAAAAACCGCACTC
TTATTCATTGATGGACAATGGTGTATACCGAAAGGAAACACCCCAACCACTCACATTATCAAGCTGCCAATAGGCGAAAT
ACCACAAGCTAACGCGACCCTGGATCTCAAAGATAGCGTAGAAAATGAATATCTTTGCATTGAGCTAGCTAAAGCGCTGG
GATTCGCAGTTCCCAGCGTCAATATAATCAAAACAGACAACCTTAAAGCTTTAGCTGTAGAACGATTTGATCGACGCTGG
ACAAAAGACAGAGCAGGCTTGCTACGCTTACCTCAAGAAGATATTTGCCAGGTGTTTGGTATGCCATCATCCATCAAATA
TGAATCTCAAGGTGGCCCAGGCATAGCACAGATCATGAAGTTATTAATGGGCTCAAGTAATGCACTTGAAGATCAATATA
ACTTCATGAGATTCCAGGTATTTCAATGGATCATTGGGGCTACGGATGGACATGCTAAAAACTTTTCTATCTATATTGAT
AGAGGAGGTAGCTATCGACTCACTCCATTCTACGACATTCTGTCGGCCTACCCATTACTTGGAGGAAAAGGACTCAATAT
TAGACAACTTAAGCTCGCAATGGGATTAAAAGCCACAAAAGGCAAGAAGTATGAAATCAATAAAATCCTGCCTCGTCACT
TTTTAGATACTGCTAAAGCTGTGAATTTTAGTCAGGATAAGATGCAAAGAATAATGGATGAAATGAAAGGTGCGTTACCT
GAGGCAATCTCGCAAGTAAGGGCGAATCTACCGGAGGGCTTTCCAGAGCATATCTCCAACTCAATTTTTGAGAATGCCAA
GAAAATAATTAAAAAAATCTAG

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT154695 WP_000806442.1 NZ_CP051661:4054304-4054645 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT154695 NZ_CP068227:c782726-782478 [Shewanella algae]
ATGATATATAGTCCAAAGCAACTCGCAGATATGATGCTACTGATACGCCAAAAGAATGGCTGGACTCAGTCAGAGTTGGC
GAAAAAAGTCGGTATAAAGCAAGCAACAATCTCGAACTTCGAGAACAACCCCAACAAAACAACGCTTTCTACCTTCTTTA
AGCTTGTACAAGCTATGGATCTAACACTGAGTATCCAAGAAAAAACACAGCTGACTTCACCAAGTGAAAATGATGATGAG
AGTTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References