Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2065749..2066548 Replicon chromosome
Accession NZ_CP051661
Organism Escherichia coli strain 1162T7

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag HH201_RS10085 Protein ID WP_000347251.1
Coordinates 2066084..2066548 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag HH201_RS10080 Protein ID WP_001296435.1
Coordinates 2065749..2066084 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HH201_RS10065 (2061534) 2061534..2062304 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
HH201_RS10070 (2062320) 2062320..2063654 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
HH201_RS10075 (2064029) 2064029..2065600 + 1572 WP_001273763.1 galactarate dehydratase -
HH201_RS10080 (2065749) 2065749..2066084 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HH201_RS10085 (2066084) 2066084..2066548 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HH201_RS10090 (2066603) 2066603..2067412 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HH201_RS10095 (2067661) 2067661..2068941 + 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
HH201_RS10100 (2068964) 2068964..2069437 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HH201_RS10105 (2069448) 2069448..2070227 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
HH201_RS10110 (2070217) 2070217..2071095 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
HH201_RS10115 (2071113) 2071113..2071547 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T154685 WP_000347251.1 NZ_CP051661:2066084-2066548 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T154685 NZ_CP068216:c804249-803887 [Staphylococcus pasteuri]
ATGATTAGAAGAGGAGATGTTTATCTAGCAGATTTATCACCTGTTCAAGGGTCCGAACAAGGGGGAGTAAGACCCGTCGT
TATTATTCAAAATGACACAGGTAATAAATATAGTCCAACAGTAATTGTTGCAGCAATTACTGGAAGGATTAACAAAGCTA
AGATACCCACACATGTAGAAATAGAAAAGAAGAAGTATAAACTTGATAAAGATTCTGTAATCTTACTTGAACAAATTCGA
ACGTTAGATAAAAATCGTTTGAAAGAAAAGCTCACATATTTGTCAGAAAACAAAATGAAAGAAGTAGATTACGCCCTAGA
CATCAGTCTAGGTTTACATAATTTTAATCATCCAAAGACTTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT154685 WP_001296435.1 NZ_CP051661:2065749-2066084 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT154685 NZ_CP068216:c804416-804246 [Staphylococcus pasteuri]
ATGTTATCTTTTAATCAAAATAGAAGTTACAGTTTAGAGCAATCTTTAAAAGAAGGTTATGCACAAATGGCTGATTTAAA
TCTCTCCCTAGCAACCGAAGCTTTTCCAATTGAATGTGAAGCGTGCGATTGCAATGAAACATATTTAACTTCTAACGCTA
AGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References