Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2024041..2024768 Replicon chromosome
Accession NZ_CP051661
Organism Escherichia coli strain 1162T7

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag HH201_RS09855 Protein ID WP_000550189.1
Coordinates 2024454..2024768 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HH201_RS09850 Protein ID WP_000560269.1
Coordinates 2024041..2024457 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HH201_RS09840 (2019401) 2019401..2021752 + 2352 WP_000695432.1 alpha-glucosidase -
HH201_RS09845 (2021978) 2021978..2023996 + 2019 WP_000121413.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
HH201_RS09850 (2024041) 2024041..2024457 - 417 WP_000560269.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
HH201_RS09855 (2024454) 2024454..2024768 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
HH201_RS09860 (2025053) 2025053..2026189 - 1137 WP_000018703.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
HH201_RS09865 (2026274) 2026274..2026777 + 504 WP_001295542.1 M48 family metallopeptidase -
HH201_RS09870 (2026854) 2026854..2027546 + 693 WP_000942551.1 vancomycin high temperature exclusion protein -
HH201_RS09875 (2027625) 2027625..2028611 + 987 WP_001385498.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T154684 WP_000550189.1 NZ_CP051661:c2024768-2024454 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T154684 NZ_CP068216:c666090-665914 [Staphylococcus pasteuri]
ATGACACAAAATAAAGATTCTAAAACTAATTATCATGAAGAAGAAAATGCTATGGTAACTGATTTAGATGATTTAAAAGA
TTTAGGTAAAGAAATGGAACAAATTTCTGAGGAAAATGATGAAGATAAATTAAACCAATCACATGATTCAGATGTTCGTT
CTGATTTAGAAGAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15023.47 Da        Isoelectric Point: 4.4547

>AT154684 WP_000560269.1 NZ_CP051661:c2024457-2024041 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFVEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT154684 NZ_CP068216:c666717-666118 [Staphylococcus pasteuri]
ATGGCAATGAACTTTAAAGTATTCGAAGATAAACAAAGAGTAGCTGAATATACAGCAGACATTTTGAGAAAACAGTTTAA
CAACAATCCTATGACTATCGCTGGTGTTCACTTAACTAAAGACCATGCACCTGTATTAGATGAACTTAAAAAGAACGTTG
AGTTACATGCAGTTGATTTTAGTCAAATTAACATTTTAGATTATGATGAAAATAAATCATACTATGAAGCTTTAGGCGTA
CCTCAAAGCCAAATTTATTCATTATCTTATAATCAAGATGCTAATGAATTTATTTCTGATAAAATTAAAACAAAAGAAAA
TAAAGGTAAATTAGTGTTACAAGTAGTTTCTATTGATGAGAATGGCAACCTTGATGTAAGCGTAAGACAAGGATTATTAG
AAGCGCGTGAAATTTTCTTAGTTGTTACTGGTAGCAATAAACGCGAAGTAGTTGAAAAATTATATAATGAAAATGGTAAG
TCTAATTATGAACCAGCAGATTTAAAAGCTCATCGCATGGTTAACGTTATTTTAGATAAAGAAGCAGCGGCTGGATTACC
TGAAGATGTTAAAGAATACTTCACTGCTCGCTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References