Detailed information of TA system TA154681

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 788117..788619
• Replicon: chromosome
• Accession: NZ_CP051661
• Organism: Escherichia coli strain 1162T7

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: HH201_RS04040
• Protein ID: WP_000767819.1
• Coordinates: 788117..788371 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: HH201_RS04045
• Protein ID: WP_001259253.1
• Coordinates: 788368..788619 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HH201_RS04015 (783935)	783935..784393	-	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-
HH201_RS04020 (784610)	784610..785968	-	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
HH201_RS04025 (785958)	785958..786020	-	63	WP_010723108.1	membrane protein YoeI	-
HH201_RS04030 (786235)	786235..787164	-	930	WP_000803366.1	LysR substrate-binding domain-containing protein	-
HH201_RS04035 (787210)	787210..788034	-	825	WP_000754737.1	SDR family oxidoreductase	-
HH201_RS04040 (788117)	788117..788371	-	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
HH201_RS04045 (788368)	788368..788619	-	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
HH201_RS04050 (788902)	788902..788952	+	51	WP_001364200.1	his operon leader peptide	-
HH201_RS04055 (789098)	789098..789997	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
HH201_RS04060 (790003)	790003..791307	+	1305	WP_001296211.1	histidinol dehydrogenase	-
HH201_RS04065 (791304)	791304..792374	+	1071	WP_000108967.1	histidinol-phosphate transaminase	-
HH201_RS04070 (792374)	792374..793441	+	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T154681 WP_000767819.1 NZ_CP051661:c788371-788117 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T154681 NZ_CP068215:c3576125-3575784 [Enterobacter hormaechei]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGCGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGCTACTGAC
CCGCCTGCTGAAACAGCACTATGGTCTCACCCTCAACGACACACCGTTCAGTGAGGAACGTGTTATACAGGAACATATCG
ATGCCGGAATCACGCTGGCTGATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGTGCGTATCGACAGGAAGGGATTT
AACTGGCAGGAACAATCCCCTTATCTCCGGGCTGTAGACATTCTGCGAGCACGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAAAAATTTGGTACGGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT154681 WP_001259253.1 NZ_CP051661:c788619-788368 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT154681 NZ_CP068215:c3576463-3576146 [Enterobacter hormaechei]
ATGACCCACATCAACTGGGGCCTGCAGCGGGCTATCACGCCGCGCCTGGGAGCCCGTCTGGTGCAGGAGGGTAACCGACT
GCACTATCTGGCTGACAGGGCCAGCATCACCGGCAGGTTCAGTGACACCGAATGCCGGAAGCTGGATGAAACATGCCCGC
ACTTTATCCGCCATATGGAGTCGATGCTAACCACCGGTGAACTCAGTCCCCAACACGCCCACTGCGTCACCCTGTACCAC
AACGGTTTCACCTGCGAAGCCGATACTCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGAGTTAA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3