Detailed information of TA system TA154668

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 32858..33127
• Replicon: plasmid pEco1162Ca
• Accession: NZ_CP051660
• Organism: Escherichia coli strain 1162C

Toxin (Protein)

• Gene name: hok
• Uniprot ID: G9G195
• Locus tag: HH202_RS24980
• Protein ID: WP_001323520.1
• Coordinates: 33011..33127 (+)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	32858..32923 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HH202_RS24945	28610..29095	+	486	WP_031311812.1	single-stranded DNA-binding protein	-
HH202_RS24950	29153..29386	+	234	WP_000006003.1	DUF905 family protein	-
HH202_RS24955	29447..31470	+	2024	Protein_47	ParB/RepB/Spo0J family partition protein	-
HH202_RS24960	31539..31973	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HH202_RS24965	31970..32732	+	763	Protein_49	plasmid SOS inhibition protein A	-
-	32701..32925	+	225	NuclAT_0	-	-
-	32701..32925	+	225	NuclAT_0	-	-
-	32701..32925	+	225	NuclAT_0	-	-
-	32701..32925	+	225	NuclAT_0	-	-
HH202_RS24970	32710..32889	-	180	WP_001309233.1	hypothetical protein	-
-	32858..32923	+	66	-	-	Antitoxin
HH202_RS24975	32911..33060	+	150	Protein_51	plasmid maintenance protein Mok	-
HH202_RS24980	33011..33127	+	117	WP_001323520.1	type I toxin-antitoxin system Hok family toxin	Toxin
HH202_RS24985	33446..33742	-	297	Protein_53	hypothetical protein	-
HH202_RS24990	34042..34338	+	297	WP_001272251.1	hypothetical protein	-
HH202_RS24995	34449..35270	+	822	WP_001234445.1	DUF932 domain-containing protein	-
HH202_RS25000	35567..36157	-	591	WP_000252683.1	transglycosylase SLT domain-containing protein	-
HH202_RS25005	36492..36875	+	384	WP_001354030.1	conjugal transfer relaxosome DNA-binding protein TraM	-
HH202_RS25010	37069..37740	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
HH202_RS25015	37877..38104	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-34)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4455.23 Da
• Isoelectric Point: 8.5110

>T154668 WP_001323520.1 NZ_CP051660:33011-33127 [Escherichia coli]
VCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 117 bp

>T154668 NZ_CP068214:c4254181-4253864 [Serratia ureilytica]
GTGCAGTTAACAGACAAAAAGAAAAGTATTGAAAGCTTTCGTGATCAGTGGCTAGATGACTTCTTCGTTACCGGTAAAGG
GCATAAGAAAATCCCTCCAGACATAGGGATTCCCTTGGGAAGGAAGCTAGACATCATAAACTCTGCGGTATCACATAAGG
ATTTACGCTCCCCGCCGGGGAATAGGTTCGAGAAGTTGAACCCACCGTTGGGAGAGTATTGCTCAATCCGGGTTAACGGT
CAGTACAGGCTGATCTTTAAGTGGATTGATGGTAAAGCAGTTGATTTATACTTAGACAACCACACATACAGAGCTTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT154668 NZ_CP051660:32858-32923 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: G9G195

Antitoxin