Detailed information of TA system TA154666

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4636016..4636618
• Replicon: chromosome
• Accession: NZ_CP051659
• Organism: Escherichia coli strain 1162C

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: HH202_RS22825
• Protein ID: WP_000897302.1
• Coordinates: 4636016..4636327 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HH202_RS22830
• Protein ID: WP_000356397.1
• Coordinates: 4636328..4636618 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HH202_RS22800 (4631930)	4631930..4632529	+	600	WP_001296610.1	glucose-1-phosphatase	-
HH202_RS22805 (4632523)	4632523..4633395	+	873	WP_000920762.1	virulence factor BrkB family protein	-
HH202_RS22810 (4633392)	4633392..4633829	+	438	WP_000560981.1	D-aminoacyl-tRNA deacylase	-
HH202_RS22815 (4633874)	4633874..4634815	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HH202_RS22820 (4634879)	4634879..4635787	-	909	WP_001385591.1	alpha/beta hydrolase	-
HH202_RS22825 (4636016)	4636016..4636327	+	312	WP_000897302.1	hypothetical protein	Toxin
HH202_RS22830 (4636328)	4636328..4636618	+	291	WP_000356397.1	NadS family protein	Antitoxin
HH202_RS22835 (4636977)	4636977..4637255	+	279	WP_001296612.1	hypothetical protein	-
HH202_RS22840 (4637652)	4637652..4637870	+	219	WP_001251293.1	CopG family transcriptional regulator	-
HH202_RS22845 (4638055)	4638055..4638795	-	741	WP_000608806.1	hypothetical protein	-
HH202_RS22850 (4638820)	4638820..4639668	-	849	WP_001038650.1	hypothetical protein	-
HH202_RS22855 (4639958)	4639958..4640200	+	243	WP_001068514.1	CopG family transcriptional regulator	-
HH202_RS22860 (4640382)	4640382..4641311	-	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T154666 WP_000897302.1 NZ_CP051659:4636016-4636327 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T154666 NZ_CP068214:3837560-3837904 [Serratia ureilytica]
ATGTGGCAAGTGGTGACCGTCGAACGATTCGACGACTGGTTTTTGGCGCTGAACAACGCCCAGCAAACAAGCATCCTGGC
GGCGATCTTCAAATTACAGACGTTCGGCCCGCAGTTGGCGCGGCCGCACGCCGATACGCTGCATTTTTCCGAAGCGGCCC
GGCAGTTGAAGGAGTTGCGCGTTCAACATCGCGGGCGCCCTTTCAGGGTGTTTTTCGCCTTTGACCCACAGCGGCAAGCA
GTATTGCTGTGCGGTGGCGATAAAACCGGCGACAAACGCTTTTACCAGCGCATGTTGCCCATCGCTGCCATGGAATTTTC
ACATTATCTGGCAACCCGGAGGTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT154666 WP_000356397.1 NZ_CP051659:4636328-4636618 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT154666 NZ_CP068214:3837910-3838212 [Serratia ureilytica]
ATGGCCAAACCGTTATCCCAACTGATCGACAAGCTCGATCCCGCCGTCGTTGCCGCAGCCAGGCAAAAAGCGGACAAGGA
AATTTTCGAACTGCGGCTGGCAATGCTGAGGGAAGAGCTGGCGGTCTCTCAGGTTGAATTGGCGAAGCGGCTCGGTATCA
GCCAACCCTCCGTCGCCAATTTGGAAAAGCGCGGCAGCGAAATAAAGCTGTCGTCGCTCAAACGCTATATCGAGGCGATG
GGCGGCACGCTGTCGCTGGATGTGCAATTGCCCAATGGCCAGCACCGTCGCATGACGCTGTGA

Structures

Toxin

• AlphaFold DB: S1P416