Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3825545..3826344 Replicon chromosome
Accession NZ_CP051659
Organism Escherichia coli strain 1162C

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag HH202_RS18870 Protein ID WP_000347251.1
Coordinates 3825880..3826344 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag HH202_RS18865 Protein ID WP_001296435.1
Coordinates 3825545..3825880 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HH202_RS18850 (3821330) 3821330..3822100 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
HH202_RS18855 (3822116) 3822116..3823450 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
HH202_RS18860 (3823825) 3823825..3825396 + 1572 WP_001273763.1 galactarate dehydratase -
HH202_RS18865 (3825545) 3825545..3825880 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HH202_RS18870 (3825880) 3825880..3826344 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HH202_RS18875 (3826399) 3826399..3827208 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HH202_RS18880 (3827457) 3827457..3828737 + 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
HH202_RS18885 (3828760) 3828760..3829233 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HH202_RS18890 (3829244) 3829244..3830023 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
HH202_RS18895 (3830013) 3830013..3830891 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
HH202_RS18900 (3830909) 3830909..3831343 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T154664 WP_000347251.1 NZ_CP051659:3825880-3826344 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T154664 NZ_CP068214:c1466002-1465718 [Serratia ureilytica]
ATGACTTATAAGCTTGAATTCGAAGAACATGCGCTGAAGGAATTTAAAAAACTGTCCCCGGTGATCCGGGAACAGTTCAA
AAAAAAGCTGGCATCGGTGCTGGTTAATCCCCACGTCCCCGCCAACAAACTCGCCGGTTTGCCCGATTGCTACAAAATCA
AACTCAGAGCCTCAGGCTTTCGTTTGGTATATCGCGTCATGGAAACGGAGATCGTGGTTCTGGTCCTGTCTGTGGGCAAA
CGCGAACGTTCAGCGGCTTACACGGCTGCCAAAAAGCGCCTTTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT154664 WP_001296435.1 NZ_CP051659:3825545-3825880 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT154664 NZ_CP068214:c1466243-1465992 [Serratia ureilytica]
ATGACGGTTCAACCCATTCTTGCCGACTCGGCTGTCAGCATCAGCGATTTTAAGAAATCGCCCAACGCCGCCCTTAAAGA
GGCACAAGGGCAACCGGTCGCCGTCCTGACCAACGGTAAGATTTCGGGATATTACGTCTCTCCGGAAACCTGGGAGGCGA
TCGCGGAATACCTGGAAGATATCGAACTCGCTGAAATCGTTCGTTCACGGATGAACGGCAAACGCATTAAGGTCAATTTG
GATGACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References