Detailed information of TA system TA154660

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2536675..2537177
• Replicon: chromosome
• Accession: NZ_CP051659
• Organism: Escherichia coli strain 1162C

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: HH202_RS12925
• Protein ID: WP_000767819.1
• Coordinates: 2536675..2536929 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: HH202_RS12930
• Protein ID: WP_001259253.1
• Coordinates: 2536926..2537177 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HH202_RS12900 (2532493)	2532493..2532951	-	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-
HH202_RS12905 (2533168)	2533168..2534526	-	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
HH202_RS12910 (2534516)	2534516..2534578	-	63	WP_010723108.1	membrane protein YoeI	-
HH202_RS12915 (2534793)	2534793..2535722	-	930	WP_000803366.1	LysR substrate-binding domain-containing protein	-
HH202_RS12920 (2535768)	2535768..2536592	-	825	WP_000754737.1	SDR family oxidoreductase	-
HH202_RS12925 (2536675)	2536675..2536929	-	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
HH202_RS12930 (2536926)	2536926..2537177	-	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
HH202_RS12935 (2537460)	2537460..2537510	+	51	WP_001364200.1	his operon leader peptide	-
HH202_RS12940 (2537656)	2537656..2538555	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
HH202_RS12945 (2538561)	2538561..2539865	+	1305	WP_001296211.1	histidinol dehydrogenase	-
HH202_RS12950 (2539862)	2539862..2540932	+	1071	WP_000108967.1	histidinol-phosphate transaminase	-
HH202_RS12955 (2540932)	2540932..2541999	+	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2532493..2532951	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T154660 WP_000767819.1 NZ_CP051659:c2536929-2536675 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T154660 NZ_CP068211:3749862-3750047 [Achromobacter xylosoxidans]
ATGAAATACAGCGAATTCAAAAAATGGCTTGAGCGGCAAGGAGCGGTTTTTGTTGCGCATCGTTCCGGAAGCAGCCATTT
TCGGGTCACGTTGAACGGCGCCACGACGATCTTTCCGTACCATGGCTCCAAGGAAATGGGTAAACACCTTGAACATGAAA
TCAAGAAGCAGCTCGGATTGAAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT154660 WP_001259253.1 NZ_CP051659:c2537177-2536926 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT154660 NZ_CP068211:3750123-3750542 [Achromobacter xylosoxidans]
ATGCTGACCTATCCGTTCACCCTGACGCTTGATTCCAATCGCACCTGGCTCGTTCGATTTCCAGATATTCCGGAAGCGTT
GACGGTTGGTGACGACGAGCAAGAGGCCGCGATCAACGCCAGGGAAGCGCTTGAGGCTGCGCTGGAAATCTACTTTGACG
CGCGCCGCCCCATTCCGCTGCCGTCGCCCGTATCGGCGGGGCAGGCGTCGGTGACCTTGCCGGCACTGATCACCAGCAAG
GTGTTCCTGTCCAACGAGATGATCCAGCAAGGTGTGCGCAAGGCGGAGTTGGCTCGCCGCATGGGCGTGCACATGCCGCA
AGTGGATCGTCTGCTGGATGTGCGGCACGCATCCCGGATCGAGCAGGTCGAGTCGGCGTTGGAGCAGCTTGGGCGGCGCC
TGGAGGTGTCGCTGGCCTGA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3