Detailed information of TA system TA154630

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2259..2683
• Replicon: plasmid pRM11911
• Accession: NZ_CP051656
• Organism: Escherichia coli strain RM11911

Toxin (Protein)

• Gene name: hok
• Uniprot ID: Q2VNZ0
• Locus tag: F7D04_RS26125
• Protein ID: WP_000775238.1
• Coordinates: 2259..2420 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	2486..2683 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D04_RS26105	1..624	+	624	WP_097368726.1	IS21-like element IS100kyp family helper ATPase IstB	-
F7D04_RS26110	691..939	-	249	Protein_1	DUF932 domain-containing protein	-
F7D04_RS26115	1058..1345	-	288	WP_000107542.1	hypothetical protein	-
F7D04_RS26120	1409..1645	+	237	Protein_3	hypothetical protein	-
F7D04_RS26125	2259..2420	-	162	WP_000775238.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2486..2683	-	198	NuclAT_0	-	Antitoxin
-	2486..2683	-	198	NuclAT_0	-	Antitoxin
-	2486..2683	-	198	NuclAT_0	-	Antitoxin
-	2486..2683	-	198	NuclAT_0	-	Antitoxin
F7D04_RS26130	2695..3414	-	720	Protein_5	plasmid SOS inhibition protein A	-
F7D04_RS26135	3411..3557	-	147	Protein_6	conjugation system SOS inhibitor PsiB	-
F7D04_RS26140	3550..3816	+	267	Protein_7	DUF4113 domain-containing protein	-
F7D04_RS26145	3818..4234	-	417	WP_001278818.1	plasmid partitioning/stability family protein	-
F7D04_RS26150	4227..5203	-	977	Protein_9	recombinase	-
F7D04_RS26155	5617..5925	-	309	WP_000030206.1	molecular chaperone GroEL	-
F7D04_RS26160	6012..6656	-	645	WP_001144036.1	ParA family protein	-
F7D04_RS26165	6835..7365	-	531	Protein_12	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyC / hlyA / hlyB / hlyD / cnf1 / cdtA / cdtB / cdtC / afaE-VIII / afaD-VIII / afaC-VIII / afaB-VIII / afaA-VIII / espP	1..175089	175089
	-	flank	IS/Tn	-	-	25..624	599

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5978.22 Da
• Isoelectric Point: 8.5101

>T154630 WP_000775238.1 NZ_CP051656:c2420-2259 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 162 bp

>T154630 NZ_CP068173:2287031-2287312 [Brevibacterium casei]
GTGATCAGATCGTTCGGCAGCAAGGAGACCGAGCGGCTGTGGCGTCGTGAGCACGTGCCCTCCCTGGACCCGCGGATCCA
GCGTGTAGCATTGCGCAAGCTTCGTCAGGTGGGCTCAGCTGAGGTACTCGAGGACCTCAGCGTTCCGCCAGGCAACCGGC
TCGAAGCGCTCAAGGGTGACCGAGCCGGTCAGCACAGCATCAGAATCAACGACCAGTGGCGGATCTGCTTCGTGTGGACC
GACGCCGGACCAGAGGAGGTGGAGATCGTTGACTACCACTGA

Antitoxin

Nucleotide

• Length: 198 bp

>AT154630 NZ_CP051656:c2683-2486 [Escherichia coli]
TCACACGGATTGCCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGC
AGAAAGCCCCGTAGTTAATTTTCATTAACCCACGAGGC

Structures

Toxin

• AlphaFold DB: A0A7Z7YI12

Antitoxin