Detailed information of TA system TA154625

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 47773..48027
• Replicon: plasmid pRM13745-2
• Accession: NZ_CP051655
• Organism: Escherichia coli strain RM13745

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: F7D05_RS26320
• Protein ID: WP_047649939.1
• Coordinates: 47821..48027 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	47773..47834 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D05_RS26290	43231..43970	+	740	Protein_43	conjugal transfer pilus acetylase TraX	-
F7D05_RS26295	44029..44888	+	860	Protein_44	alpha/beta hydrolase	-
F7D05_RS26300	44991..45548	+	558	WP_000139319.1	fertility inhibition protein FinO	-
F7D05_RS26305	45703..45906	+	204	WP_001327131.1	hypothetical protein	-
F7D05_RS26310	46651..47112	+	462	WP_000760078.1	thermonuclease family protein	-
F7D05_RS26315	47215..47598	+	384	WP_001109066.1	hypothetical protein	-
-	47773..47834	-	62	NuclAT_0	-	Antitoxin
-	47773..47834	-	62	NuclAT_0	-	Antitoxin
-	47773..47834	-	62	NuclAT_0	-	Antitoxin
-	47773..47834	-	62	NuclAT_0	-	Antitoxin
F7D05_RS26320	47821..48027	+	207	WP_047649939.1	type I toxin-antitoxin system Hok family toxin	Toxin
F7D05_RS26325	48307..48555	+	249	WP_000083838.1	replication regulatory protein RepA	-
F7D05_RS26330	48802..48876	+	75	WP_001365705.1	RepA leader peptide Tap	-
F7D05_RS26335	48869..49741	+	873	WP_151316381.1	incFII family plasmid replication initiator RepA	-
F7D05_RS26340	50849..51745	-	897	Protein_53	IS3 family transposase	-
F7D05_RS26345	51815..52819	+	1005	WP_050939068.1	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hlyC / hlyA / hlyB / hlyB / hlyD / hlyD	1..70650	70650

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7762.27 Da
• Isoelectric Point: 8.8807

>T154625 WP_047649939.1 NZ_CP051655:47821-48027 [Escherichia coli]
MKYLNTTDCSLFLAERSKFMTKYALIGGLAVCATVLCFLLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T154625 NZ_CP068166:278982-279239 [Corynebacterium aurimucosum]
ATGAAGACCATGTCTTACTCGGAATCGAGAGCACGGTACGCCGAAGTATTGCAGTCGGTTGTTGATGACCGCGAGGAAGT
CATCATCACTCGCAATGGTCATGAGTCTGCAGTCATCGTTTCCTTCGATGACTACAACTCTCTTCGCGAAACTGCTTACC
TTCTGCGTTCCCCTCGCAATGCCCAGCGCCTACTTGACTCCATTGAGAGCCTCAATACGGGGAAAGCAACGGAACACGAG
CTCATTGAGGAAGATTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT154625 NZ_CP051655:c47834-47773 [Escherichia coli]
TTAAGGTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin