Detailed information of TA system TA154618

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 69905..70159
• Replicon: plasmid pRM13752
• Accession: NZ_CP051653
• Organism: Escherichia coli strain RM13752

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: F7D06_RS26180
• Protein ID: WP_047649939.1
• Coordinates: 69953..70159 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	69905..69966 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D06_RS26150	65361..66101	+	741	WP_050939821.1	conjugal transfer pilus acetylase TraX	-
F7D06_RS26155	66160..67020	+	861	WP_000868986.1	alpha/beta hydrolase	-
F7D06_RS26160	67123..67680	+	558	WP_000139319.1	fertility inhibition protein FinO	-
F7D06_RS26165	67835..68038	+	204	WP_001327131.1	hypothetical protein	-
F7D06_RS26170	68783..69244	+	462	WP_000760078.1	thermonuclease family protein	-
F7D06_RS26175	69347..69730	+	384	WP_001109066.1	hypothetical protein	-
-	69905..69966	-	62	NuclAT_0	-	Antitoxin
-	69905..69966	-	62	NuclAT_0	-	Antitoxin
-	69905..69966	-	62	NuclAT_0	-	Antitoxin
-	69905..69966	-	62	NuclAT_0	-	Antitoxin
F7D06_RS26180	69953..70159	+	207	WP_047649939.1	type I toxin-antitoxin system Hok family toxin	Toxin
F7D06_RS26185	70440..70688	+	249	WP_000083838.1	replication regulatory protein RepA	-
F7D06_RS26190	70935..71009	+	75	WP_001365705.1	RepA leader peptide Tap	-
F7D06_RS26195	71002..71859	+	858	WP_000131011.1	incFII family plasmid replication initiator RepA	-
F7D06_RS26200	72774..72950	+	177	WP_157905840.1	hypothetical protein	-
F7D06_RS26205	72983..73879	-	897	Protein_79	IS3 family transposase	-
F7D06_RS26210	73949..74953	+	1005	WP_050939068.1	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	espP / hlyC / hlyA / hlyB / hlyD / cnf1	1..99009	99009

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7762.27 Da
• Isoelectric Point: 8.8807

>T154618 WP_047649939.1 NZ_CP051653:69953-70159 [Escherichia coli]
MKYLNTTDCSLFLAERSKFMTKYALIGGLAVCATVLCFLLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T154618 NZ_CP068159:c2214241-2213987 [Corynebacterium riegelii]
ATGCGGCTGGTTTGGACTAGCCACGCGTGGGAAGACTATTTGCATTGGCAGGCCAATGACAAAAAGATGCTCAAACGCGT
CAATCAGCTGCTGCGTGATACGCTGCGTGACCCTTTTGAGGGGATAGGCAAGCCTGAGCCTTTGAAATATGGTGCTGAGG
GTGCTTGGTCACGAAGAATTACGCAAGAACACCGACTGGTCTACATGATTGTTGACGGGGATCTTGTAGTCTTGCAGGCG
CGTTACCACTACTAG

Antitoxin

Nucleotide

• Length: 62 bp

>AT154618 NZ_CP051653:c69966-69905 [Escherichia coli]
TTAAGGTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin