Detailed information of TA system TA154602

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4397620..4398209
• Replicon: chromosome
• Accession: NZ_CP051651
• Organism: Xanthomonas campestris pv. badrii strain NEB122

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: HG421_RS18690
• Protein ID: WP_169707657.1
• Coordinates: 4397928..4398209 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HG421_RS18685
• Protein ID: WP_169707656.1
• Coordinates: 4397620..4397910 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HG421_RS18655	4392884..4393771	+	888	WP_169707652.1	hypothetical protein	-
HG421_RS18660	4393768..4394430	+	663	WP_169707653.1	hypothetical protein	-
HG421_RS18665	4394587..4395954	+	1368	WP_168968245.1	IS5 family transposase	-
HG421_RS18670	4396088..4396420	+	333	WP_169707654.1	hypothetical protein	-
HG421_RS18675	4397026..4397280	+	255	WP_169707655.1	antitoxin	-
HG421_RS18680	4397280..4397583	+	304	Protein_3628	type II toxin-antitoxin system RelE/ParE family toxin	-
HG421_RS18685	4397620..4397910	-	291	WP_169707656.1	HigA family addiction module antidote protein	Antitoxin
HG421_RS18690	4397928..4398209	-	282	WP_169707657.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HG421_RS18695	4398349..4400709	-	2361	WP_169707658.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4390083..4400709	10626
	-	flank	IS/Tn	-	-	4394587..4395954	1367

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11082.80 Da
• Isoelectric Point: 10.2121

>T154602 WP_169707657.1 NZ_CP051651:c4398209-4397928 [Xanthomonas campestris pv. badrii]
MIRSFIDKEAEKIWLGERSRRLPAHIQSVARRKLRMLNAAAHLDDLRIPPANRLEALKGERRGQYSIRINDQWRICFRWM
EGDVAEVEIVDYH

Nucleotide

• Length: 282 bp

>T154602 NZ_CP068155:c1743612-1743439 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10450.10 Da
• Isoelectric Point: 7.2043

>AT154602 WP_169707656.1 NZ_CP051651:c4397910-4397620 [Xanthomonas campestris pv. badrii]
MTVLPNIHPGEILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPSRIKRLAA

Nucleotide

• Length: 291 bp

>AT154602 NZ_CP068155:c1743936-1743640 [Escherichia coli]
ATGGAAGGTAAAAACAGGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG