Detailed information of TA system TA154518

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 1656680..1657306
• Replicon: chromosome
• Accession: NZ_CP051633
• Organism: Treponema denticola strain OMZ 898

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q73NN6
• Locus tag: E4N85_RS07530
• Protein ID: WP_002677634.1
• Coordinates: 1656680..1656988 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E4N85_RS07535
• Protein ID: WP_002670672.1
• Coordinates: 1656998..1657306 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4N85_RS07505 (E4N85_07555)	1652635..1652973	+	339	WP_002670686.1	anti-sigma factor antagonist	-
E4N85_RS07510 (E4N85_07560)	1652990..1653532	+	543	WP_002670683.1	ATP-binding protein	-
E4N85_RS07515 (E4N85_07565)	1653547..1654368	-	822	WP_253689869.1	hypothetical protein	-
E4N85_RS07520 (E4N85_07570)	1654349..1654969	-	621	WP_002671735.1	hypothetical protein	-
E4N85_RS07525 (E4N85_07575)	1655085..1656464	+	1380	WP_002670676.1	tyrosine phenol-lyase	-
E4N85_RS07530 (E4N85_07580)	1656680..1656988	+	309	WP_002677634.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4N85_RS07535 (E4N85_07585)	1656998..1657306	+	309	WP_002670672.1	NadS family protein	Antitoxin
E4N85_RS07540 (E4N85_07590)	1657439..1658110	+	672	WP_002670670.1	TetR/AcrR family transcriptional regulator	-
E4N85_RS07545 (E4N85_07595)	1658199..1658615	+	417	WP_245522456.1	SRPBCC family protein	-
E4N85_RS07550 (E4N85_07600)	1658747..1659376	-	630	WP_002670667.1	adenylate kinase	-
E4N85_RS07555 (E4N85_07605)	1659435..1660664	-	1230	WP_002670666.1	cation:dicarboxylase symporter family transporter	-
E4N85_RS07560 (E4N85_07610)	1660703..1662022	-	1320	WP_002670665.1	leucine-rich repeat domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-86)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11736.96 Da
• Isoelectric Point: 10.6864

>T154518 WP_002677634.1 NZ_CP051633:1656680-1656988 [Treponema denticola]
MRIIETPVFTKQINRLLDEDTYKQFKEYLVCNPLKGKLIKGGGGIRKIRWSKKNTGKSGGIRIIYFIKTETKIYLLFAYS
KSDAESITKKQINMLASFIKGL

Nucleotide

• Length: 309 bp

>T154518 NZ_CP068139:2976683-2977051 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCTGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11533.43 Da
• Isoelectric Point: 9.6884

>AT154518 WP_002670672.1 NZ_CP051633:1656998-1657306 [Treponema denticola]
MFKRKKMSDEMFAELLQSVKEAVLIEKGEIPPARVFEIEPLDIAKIRSKTNKTQEEFASMLNISIGTLRNWEQGRRKPDG
AALSLLKIVSANPQYVESVLQG

Nucleotide

• Length: 309 bp

>AT154518 NZ_CP068139:2976465-2976686 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: Q73NN6