Detailed information of TA system TA154514

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2310191..2310780
• Replicon: chromosome
• Accession: NZ_CP051630
• Organism: Pyruvatibacter mobilis strain CGMCC 1.15125

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: HG718_RS10755
• Protein ID: WP_160588436.1
• Coordinates: 2310493..2310780 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HG718_RS10750
• Protein ID: WP_160588435.1
• Coordinates: 2310191..2310487 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HG718_RS10745	2308362..2310194	+	1833	WP_160588514.1	DNA helicase RecQ	-
HG718_RS10750	2310191..2310487	-	297	WP_160588435.1	HigA family addiction module antidote protein	Antitoxin
HG718_RS10755	2310493..2310780	-	288	WP_160588436.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HG718_RS10760	2310837..2311208	-	372	WP_160588437.1	VOC family protein	-
HG718_RS10765	2311431..2312783	+	1353	WP_160588438.1	hypothetical protein	-
HG718_RS10770	2312894..2313763	-	870	WP_160588439.1	TauD/TfdA family dioxygenase	-
HG718_RS10775	2314030..2314494	-	465	WP_160588440.1	NfeD family protein	-
HG718_RS10780	2314508..2315509	-	1002	WP_160588441.1	SPFH/Band 7/PHB domain protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-66)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11136.70 Da
• Isoelectric Point: 10.2719

>T154514 WP_160588436.1 NZ_CP051630:c2310780-2310493 [Pyruvatibacter mobilis]
MAIRSFSSRETEKLYLGERSRKFGALSRKAMLQLRRIDAAHVLEDLRVPPGNHLEALKGDRRGQHSIRIDRQWRICFRWD
AGDAYDVEIVDYHKG

Nucleotide

• Length: 288 bp

>T154514 NZ_CP068139:1365509-1365727 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10382.09 Da
• Isoelectric Point: 10.2710

>AT154514 WP_160588435.1 NZ_CP051630:c2310487-2310191 [Pyruvatibacter mobilis]
MARTPMHPGEMLKEGFLDELGLSATSLARHIGVPANRLTSIIAGKRGITGETAILLGKALGTTPEFWLNLQKTYELDVAA
TSDVAKKARSIRPVAPAA

Nucleotide

• Length: 297 bp

>AT154514 NZ_CP068139:1365108-1365482 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG