Detailed information of TA system TA154501

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 94463..94705
• Replicon: plasmid pAPHA_2017_1
• Accession: NZ_CP051616
• Organism: Escherichia coli O25b:H4-ST131 strain 2017_APHA

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HHM36_RS24575
• Protein ID: WP_001312861.1
• Coordinates: 94463..94621 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	94665..94705 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHM36_RS24535	89575..89802	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
HHM36_RS24540	89890..90567	-	678	WP_001348626.1	PAS domain-containing protein	-
HHM36_RS24545	90701..91084	-	384	WP_001151566.1	relaxosome protein TraM	-
HHM36_RS24550	91415..92017	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
HHM36_RS24555	92314..93135	-	822	WP_001234469.1	DUF945 domain-containing protein	-
HHM36_RS24560	93254..93541	-	288	WP_000107535.1	hypothetical protein	-
HHM36_RS24565	93566..93772	-	207	WP_000275859.1	hypothetical protein	-
HHM36_RS24570	93685..94020	-	336	WP_013023876.1	hypothetical protein	-
HHM36_RS24575	94463..94621	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	94665..94705	-	41	NuclAT_1	-	Antitoxin
-	94665..94705	-	41	NuclAT_1	-	Antitoxin
-	94665..94705	-	41	NuclAT_1	-	Antitoxin
-	94665..94705	-	41	NuclAT_1	-	Antitoxin
-	96149..96335	-	187	NuclAT_0	-	-
-	96149..96335	-	187	NuclAT_0	-	-
-	96149..96335	-	187	NuclAT_0	-	-
-	96149..96335	-	187	NuclAT_0	-	-
HHM36_RS24585	96347..97066	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
HHM36_RS24590	97063..97497	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
HHM36_RS24595	97552..99510	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA17 / aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaCTX-M-27	senB	1..134872	134872

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T154501 WP_001312861.1 NZ_CP051616:c94621-94463 [Escherichia coli O25b:H4-ST131]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T154501 NZ_CP068117:37380-37649 [Enterococcus hirae]
TTGGGATTCACTATAGAGATTAAGAAAAATGCCAAAAAGGATTTACCAAAATTGAAAGCAGCAGGATTGCATAAAAAATT
TGATGAACTGATTCAAGTGATTCGAGAAAATCCTTTTCAAACTCCACCACCATATGAAAAATTAGTTGGTGTTCCATATT
ATTCAAGAAGAATTAATATACAGCACCGATTAGTTTATAGCGTAGATACAGAAGAAAATGTGGTAGTTATCCTTTCTATA
TGGTCACAATATGAAAGAGGATTAAATTAG

Antitoxin

Nucleotide

• Length: 41 bp

>AT154501 NZ_CP051616:c94705-94665 [Escherichia coli O25b:H4-ST131]
TTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin