Detailed information of TA system TA154497

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 66969..67208
• Replicon: plasmid pAPHA_2017_1
• Accession: NZ_CP051616
• Organism: Escherichia coli O25b:H4-ST131 strain 2017_APHA

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: HHM36_RS24420
• Protein ID: WP_023144756.1
• Coordinates: 66969..67103 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	67148..67208 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHM36_RS24385	62316..62731	-	416	Protein_73	IS1 family transposase	-
HHM36_RS24390	62980..63381	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
HHM36_RS24395	63314..63571	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
HHM36_RS24400	63664..64317	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
HHM36_RS24405	65257..66114	-	858	WP_001617855.1	incFII family plasmid replication initiator RepA	-
HHM36_RS24410	66107..66181	-	75	WP_031943482.1	RepA leader peptide Tap	-
HHM36_RS24415	66418..66672	-	255	WP_000083850.1	replication regulatory protein RepA	-
HHM36_RS24420	66969..67103	-	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	67148..67208	+	61	NuclAT_2	-	Antitoxin
-	67148..67208	+	61	NuclAT_2	-	Antitoxin
-	67148..67208	+	61	NuclAT_2	-	Antitoxin
-	67148..67208	+	61	NuclAT_2	-	Antitoxin
HHM36_RS24425	67175..67461	-	287	Protein_81	DUF2726 domain-containing protein	-
HHM36_RS24430	67539..69152	-	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
HHM36_RS24435	69183..69533	-	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
HHM36_RS24440	69530..69955	-	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
HHM36_RS24445	70514..70726	-	213	WP_013023861.1	hypothetical protein	-
HHM36_RS24450	70857..71417	-	561	WP_000139341.1	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA17 / aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaCTX-M-27	senB	1..134872	134872

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T154497 WP_023144756.1 NZ_CP051616:c67103-66969 [Escherichia coli O25b:H4-ST131]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T154497 NZ_CP068112:c130894-130586 [Mobiluncus curtisii]
GTGGAGATTATTTCTAGTAGCTTGTTTGACGCTTGGTTAGGTGAGCTGAAAGACAAACATGCTCAGCGGCGCATTTTGCA
TGCAATAGCCCGATGCGAAGCACACGGAACAATGGTTGGAGACATCAAACCCGTTGGCGAGAAAGTTAACGAGATGAGAT
TTCATTTCGGGCCCGGCTACCGGGTGTACTACACCCAACTAGGTGCAGTAACGGTTTTCCTGCTGGCCGGAGGAGATAAA
TCCAGCCAGGCTAAAGACATTCGAACCGCTCAAAAGCTCGCACAACAGGTAAGGAAGGAACAGTCATGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT154497 NZ_CP051616:67148-67208 [Escherichia coli O25b:H4-ST131]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin