Detailed information of TA system TA154492

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 4094812..4095491
• Replicon: chromosome
• Accession: NZ_CP051615
• Organism: Escherichia coli O25b:H4-ST131 strain 2017_APHA

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1PK60
• Locus tag: HHM36_RS19445
• Protein ID: WP_000057523.1
• Coordinates: 4094812..4095114 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: S1QAY3
• Locus tag: HHM36_RS19450
• Protein ID: WP_000806442.1
• Coordinates: 4095150..4095491 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHM36_RS19420	4090188..4091840	+	1653	WP_000771748.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-
HHM36_RS19425	4091878..4092381	-	504	WP_000667000.1	hypothetical protein	-
HHM36_RS19430	4092378..4093178	-	801	WP_000439798.1	hypothetical protein	-
HHM36_RS19435	4093202..4093681	-	480	WP_000186633.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
HHM36_RS19440	4093885..4094679	-	795	WP_000365147.1	TraB/GumN family protein	-
HHM36_RS19445	4094812..4095114	+	303	WP_000057523.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HHM36_RS19450	4095150..4095491	+	342	WP_000806442.1	HigA family addiction module antidote protein	Antitoxin
HHM36_RS19455	4095549..4098053	-	2505	WP_000083947.1	copper-exporting P-type ATPase CopA	-
HHM36_RS19460	4098315..4099247	+	933	WP_000883041.1	glutaminase A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-100)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11809.44 Da
• Isoelectric Point: 10.3980

>T154492 WP_000057523.1 NZ_CP051615:4094812-4095114 [Escherichia coli O25b:H4-ST131]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Nucleotide

• Length: 303 bp

>T154492 NZ_CP068102:2208375-2208743 [Rothia aeria]
ATGATCCTCGTTGACACTAATGTGTGGATAGATCATCTGCATCATGCAGAACCCGAACTGTCGGAGCTTCTAATACGTGA
TGAAATCTGCTGTCATGATTTAGTGCTCACAGAATTAGCCCTTGGTTCCCTTAAAAAACGAGATGAGTTTCTATCTGCTC
TGAGCGAGCTGAAACGGCTTCCTGCAGTCGATGATCACGAAGTGCGGTGGTTTATCGAAAAACGTAAGCTGTGGGGACGC
GGTTTATCTGCTGTTGATGTGTACCTTTTAGCCTCGGTTGTGGTAGTTCCAGGAACTACTCTGTGGACTCGTGACAAACG
TTTGCGCGATGTGGCACAAGACCTGGGGTGTCGGTTCGAGAACGGCTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13171.10 Da
• Isoelectric Point: 5.7790

>AT154492 WP_000806442.1 NZ_CP051615:4095150-4095491 [Escherichia coli O25b:H4-ST131]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Nucleotide

• Length: 342 bp

>AT154492 NZ_CP068102:2208181-2208378 [Rothia aeria]
ATGCGTACAACCGTTACCCTTGACGAAGAAATCGTTGCTAAAGCCGTCAAACTCACCGGTATCGAAGAGCGCTCAGTGTT
AGTGCGCGAAGCTATGGAAACTCTGGTACGTATCGAAAACGGGCGTTTGCTTGCCGCTTTAGGTGGCTCCGATCCCCAGG
CCCAAGCGGCTCCGCGGCATCGAAATGTGCAGTCATGA

Structures

Toxin

• AlphaFold DB: S1PK60

Antitoxin

• AlphaFold DB: S1QAY3